JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 22: Issue 3
Validated HPTLC method of analysis of dexibuprofen in its formulation
Authors: Selvadurai Muralidharan 1 , Subramania Meyyanathan 1 , Nithyanantham Muruganantham 2 , Sekar Rajan 1 , Kaliaperumal Krishnaraj 2 , and Bhojraj Suresh 3
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  • 1 JSS College of Pharmacy Department of Pharmaceutical Analysis Rocklands, Ooty, Ootacamund 643 001 India
  • 2 JSS College of Pharmacy TIFAC CORE Rocklands, Ooty, Ootacamund 643 001 India
  • 3 JSS College of Pharmacy Rocklands, Ooty, Ootacamund 643 001 India
DOI:
https://doi.org/10.1556/jpc.22.2009.3.9
Page Count:
207–210
Publication Date:
01 Jun 2009
Online Publication Date:
18 Jun 2009
Article Category:
Research Article
Restricted access

A simple, sensitive, precise, and rapid high-performance thin-layer chromatographic (HPTLC) method for analysis of dexibuprofen in pharmaceutical formulation has been developed and validated. The method uses aluminum foil HPTLC plates coated with silica gel 60F 254 as stationary phase and hexane-ethyl acetate-glacial acetic acid 7.5:2.5:0.5 ( v/v ) as mobile phase. Densitometric analysis of dexibuprofen and the internal standard (aceclofenac) was performed in reflectance mode at 217 nm. The system was found to give compact bands for dexibuprofen ( R F 0.50). Linear regression analysis of the calibration data revealed a good linear relationship between response and concentration in the range 50–300 ng per band ( r 2 = 0.9902). The method was validated for precision, accuracy, recovery, and robustness. The method has been successfully applied to analysis of an oral solid dosage formulation.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993
Keywords:
Dexibuprofen; HPTLC; Formulation; Validation

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