JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 22: Issue 4
High-performance thin-layer chromatography combined with densitometry for quantitative analysis of chlorogenic acid in fruits of Peucedanum alsaticum L.
Authors:
Krystyna Skalicka-Woźniak Medical University of Lublin Department of Pharmacognosy with Medicinal Plant Laboratory Chodzki 1 20-093 Lublin Poland

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Michał Hajnos Medical University of Lublin Department of Pharmacognosy with Medicinal Plant Laboratory Chodzki 1 20-093 Lublin Poland

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Kazimierz Głowniak Medical University of Lublin Department of Pharmacognosy with Medicinal Plant Laboratory Chodzki 1 20-093 Lublin Poland

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Pages:
297–300
Online Publication Date:
09 Oct 2009
Publication Date:
01 Aug 2009
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.22.2009.4.11
Keywords:
High-performance thin-layer chromatography; Densitometry; Peucedanum alsaticum ; Chlorogenic acid
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High-performance thin-layer chromatography with densitometric detection has been used for quantitative analysis of chlorogenic acid in extracts obtained by different methods from the fruits of Peucedanum alsaticum . Separation was achieved on silica gel HPTLC plates with ethyl acetate-formic acid-acetic acid-water 100:11:11:21 as mobile phase. Densitometric detection was performed before and after derivatization with Naturstoffreagenz A at 320 nm. Calibration plots were linear in the range 10–75 μg mL −1 . The results obtained were very similar to those from HPLC, so this densitometric method can be easily used for easy and economic quantitative analysis of active compounds in plant material.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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