HPTLC has been used for quantitative analysis of aloin, as marker compound, in commercial Ayurvedic preparations, in the solid dosage form, containing
Linn as major herbal ingredient. Chromatography was performed on aluminum plates coated with 0.2-mm layers of silica gel 60 F
, with ethyl acetate-methanol-water 10:1.4:1 (
) as ternary mobile phase after saturation at 30 ± 4°C for 5–7 min. The development distance was 70 mm. Aloin was quantified at 360 nm, its wave length of maximum absorbance. The limits of detection and quantification were 10 and 20 ng per band, respectively. Regression analysis of the calibration data revealed a good linear relationship (
= 0.9998) between peak area response and concentration in the range 20 to 100 ng per band. Instrumental precision and the repeatability of the method were 0.44% and 0.89% (CV), respectively. The accuracy of the method, determined by measurement of recovery at three different levels, was in the range 98.13 to 99.75%. These results are indicative of the excellent reliability, reproducibility, accuracy, and precision of the method. The method has been successfully used for analysis of aloin in commercial formulations containing
Calixto J.B., '' (2000) 33Brazil. J. Med. Bio. Res.: 179-189.
Calixto J.B.Brazil. J. Med. Bio. Res.200033179189)| false
J. Singh, A.K. Sing, S.P.S. Khanuja
, Pharma Bio World
Khanuja S.P.S., '' (2003) 1Pharma Bio World: 59-66.
Khanuja S.P.S.Pharma Bio World200315966)| false
Research guidelines for evaluating the safety and efficacy of herbal medicines, WHO, Regional Office for the Western Pacific, Manila, 1993.
, Proceedings of the International Congress on Ayurveda, 28–30 January, 2002, Kochi, Kerala, India, pp. 67.
The Ayurvedic formulary of India, first edn., Part I, Ministry of Health and Family Planning, Dept. of Health, Govt. of India, 1978, pp. 178.
Pharmacopoeial Standards of Ayurvedic Formulations, revised edn., Central Council for Research in Ayurveda & Siddha, Govt. of India, New Delhi, 1978, pp. 420–423.
The Ayurvedic Pharmacopoeia of India, Part II, Vol. I (Formulations), Ministry of Health and Family Welfare, Department of Ayurveda, Yoga & Naturopathy, Unani, Siddha and Homoeopathy, Government of India, New Delhi, 2007.
S. Agarwal, R.H. Singh
, Proceedings of the International Congress on Ayurveda, 28–30th January, 2002, Kochi, Kerala, India, Abstract No. 209, pp. 221.
A.T. Elamthuruthy, C.R. Shah, T.A. Khan, P.A. Tatke, S.Y. Gabhe
, J. Pharm. Biomed. Anal.