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  • 1 Utkal University Pharmacognosy and Phytochemistry Division, University Department of Pharmaceutical Sciences Vani Vihar, Bhubaneswar 751004 Orissa India
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HPTLC has been used for quantitative analysis of aloin, as marker compound, in commercial Ayurvedic preparations, in the solid dosage form, containing Aloe vera Linn as major herbal ingredient. Chromatography was performed on aluminum plates coated with 0.2-mm layers of silica gel 60 F 254 , with ethyl acetate-methanol-water 10:1.4:1 ( v/v ) as ternary mobile phase after saturation at 30 ± 4°C for 5–7 min. The development distance was 70 mm. Aloin was quantified at 360 nm, its wave length of maximum absorbance. The limits of detection and quantification were 10 and 20 ng per band, respectively. Regression analysis of the calibration data revealed a good linear relationship ( r = 0.9998) between peak area response and concentration in the range 20 to 100 ng per band. Instrumental precision and the repeatability of the method were 0.44% and 0.89% (CV), respectively. The accuracy of the method, determined by measurement of recovery at three different levels, was in the range 98.13 to 99.75%. These results are indicative of the excellent reliability, reproducibility, accuracy, and precision of the method. The method has been successfully used for analysis of aloin in commercial formulations containing Aloe vera .

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