A new simple, accurate, and precise densitometric method for determination of olmesartan medoxomil (OM) and hydrochlorothiazide (HTZ) in combined tablet dosage forms has been developed and validated. Separation of the drugs was carried out using chloroform-methanol-toluene 6:4:5 (
) as mobile phase on precoated silica gel 60 F
plates. The retention factors for HTZ and OM were 0.40 ± 0.019 and 0.58 ± 0.013, respectively. The detection of bands was carried out at 258 nm. The calibration curve was linear in the concentration range 100 to 600 ng per band for OM and 50 to 300 ng per band for HTZ. For OM, the recovery study results ranged from 99.92 to 100.82% with RSD values ranging from 0.300 to 0.851%. For HTZ, the recovery results ranged from 99.21 to 100.34% with RSD values ranging from 0.203 to 0.489%. The assay [%] was 99.989 ± 0.389 and 99.516 ± 0.303 for OM and 100.35 ± 0.609 and 100.17 ± 0.595 for HTZ (mean ± S.D.,
= 6), respectively, in two different tablet formulations tested. The method can be used for routine analysis of these drugs in combined tablet dosage forms in quality-control laboratories.