Carotenoids are important pigments found in foods and biological samples. Among carotenoids, β-carotene is the major carotenoid present in vegetable oils. It plays an important role in the thermal stability of the vegetable oil. We established a simple, precise, specific, sensitive, repeatable, and accurate HPTLC method for the analysis of β-carotene in fortified vegetable oils and assessment of its degradation. Analysis was performed on silica gel HPTLC plates with petroleum ether-hexane-acetone 2:3:1 (
) as mobile phase and densitometric detection. The
of β-carotene was 0.91 and regression analysis showed response was a linear function quantification of amount of β-carotene in the range 100–600 ng (
= 0.99991). The limits of detection and quantification were 0.11 and 0.37 ng, respectively. The thermal degradation (1–5 h at 100°C) of β-carotene in fortified sunflower oil was studied. It was observed that this HPTLC method could be used for efficient analysis and monitoring of the degradation of β-carotene in edible oils.
G. Tang, J. Qin, G.G. Dolnikowski, R.M. Russell
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Russell R.M., '' (2000) 39Eur. J. Nutr.: 7-11.
Russell R.M.Eur. J. Nutr.200039711)| false
J. von-Lintig, K. Vogt
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Vogt K., '' (2000) 275J. Bio. Chem.: 11915-11920.
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S.A.R. Paiva, R.M. Russell
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Russell R.M.J. Am. Coll. Nutr.199918426433)| false
M.A. Montenegro, M.A. Nazareno, E.N. Durantini, E.N. Borsarelli
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