A simple, rapid, and effective high-performance thin-layer chromatographic (HPTLC) method has been established for differentiating among the polysaccharides present in six traditional Chinese medicines (TCM),
Cordyceps sinensis, Ganoderma lucidum, Astragalus memberanaceus, Panax ginseng, Panax quinquefolii
. Acid hydrolyzates of the polysaccharides were analyzed by HPTLC with two detection reagents, aniline-diphenylamine-phosphoric acid and ninhydrin, and scanning densitometry. The compounds were separated on silica gel plates with chloroform-
-butanol-methanol-acetic acid-water 4.5:12.5:5:1.5:1.5 (
) as mobile phase. Seven monosaccharides and two glucuronic acids were used as reference compounds. The results showed that hydrolysis of polysaccharides can release specific molecules present in the herbal species in addition to the monosaccharides present. This is useful for distinguishing the origins of the polysaccharides in Chinese medicines.
J. Guan, S.P. Li
(ed.), Chinese Herbal Drug Research Trends, Nova Science Publishers, NewYork, 2007, p. 31–65.
Li S.P., '', in Chinese Herbal Drug Research Trends, (2007) -.
Li S.P.Chinese Herbal Drug Research Trends2007)| false
X. Di, K.K.C. Chan, H.W. Leung, C.W. Huie
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Huie C.W., '' (2003) 1018J. Chromatogr. A: 85-95.
Huie C.W.J. Chromatogr. A200310188595)| false
G.L. Sassaki, L.M.D. Souza, T.R. Cipriani, M. Iacomini, M. Iacomini
M. Waksmundzka-Hajnos, J. Sherma, and T. Kowalska
(eds.), Thin Layer Chromatography in Phytochemistry, CRC Press, Boca Raton, 2008, p. 255–276.
Iacomini M., '', in Thin Layer Chromatography in Phytochemistry, (2008) -.
Iacomini M.Thin Layer Chromatography in Phytochemistry2008)| false