JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 23: Issue 1
Use of HPTLC to differentiate among the crude polysaccharides in six traditional Chinese medicines
Authors:
Cheng Yang University of Macau Institute of Chinese Medical Sciences Macau SAR China

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Jia Guan University of Macau Institute of Chinese Medical Sciences Macau SAR China

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Jiang-sheng Zhang University of Macau Institute of Chinese Medical Sciences Macau SAR China

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Shao-ping Li University of Macau Institute of Chinese Medical Sciences Macau SAR China

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Pages:
46–49
Online Publication Date:
19 Mar 2010
Publication Date:
01 Feb 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.23.2010.1.8
Keywords:
Polysaccharides; HPTLC; Quality control; Traditional Chinese medicines (TCM)
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A simple, rapid, and effective high-performance thin-layer chromatographic (HPTLC) method has been established for differentiating among the polysaccharides present in six traditional Chinese medicines (TCM), Cordyceps sinensis, Ganoderma lucidum, Astragalus memberanaceus, Panax ginseng, Panax quinquefolii , and Panax notogiseng . Acid hydrolyzates of the polysaccharides were analyzed by HPTLC with two detection reagents, aniline-diphenylamine-phosphoric acid and ninhydrin, and scanning densitometry. The compounds were separated on silica gel plates with chloroform- n -butanol-methanol-acetic acid-water 4.5:12.5:5:1.5:1.5 ( v/v ) as mobile phase. Seven monosaccharides and two glucuronic acids were used as reference compounds. The results showed that hydrolysis of polysaccharides can release specific molecules present in the herbal species in addition to the monosaccharides present. This is useful for distinguishing the origins of the polysaccharides in Chinese medicines.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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