Authors:
Cheng YangUniversity of Macau Institute of Chinese Medical Sciences Macau SAR China

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Jia GuanUniversity of Macau Institute of Chinese Medical Sciences Macau SAR China

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Jiang-sheng ZhangUniversity of Macau Institute of Chinese Medical Sciences Macau SAR China

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Shao-ping LiUniversity of Macau Institute of Chinese Medical Sciences Macau SAR China

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A simple, rapid, and effective high-performance thin-layer chromatographic (HPTLC) method has been established for differentiating among the polysaccharides present in six traditional Chinese medicines (TCM), Cordyceps sinensis, Ganoderma lucidum, Astragalus memberanaceus, Panax ginseng, Panax quinquefolii , and Panax notogiseng . Acid hydrolyzates of the polysaccharides were analyzed by HPTLC with two detection reagents, aniline-diphenylamine-phosphoric acid and ninhydrin, and scanning densitometry. The compounds were separated on silica gel plates with chloroform- n -butanol-methanol-acetic acid-water 4.5:12.5:5:1.5:1.5 ( v/v ) as mobile phase. Seven monosaccharides and two glucuronic acids were used as reference compounds. The results showed that hydrolysis of polysaccharides can release specific molecules present in the herbal species in addition to the monosaccharides present. This is useful for distinguishing the origins of the polysaccharides in Chinese medicines.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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