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A high-performance thin-layer chromatographic (HPTLC) method has been established for quantitative analysis of andrographolide, the major bioactive constituent of the immunomodulatory plant Andrographis paniculata Nees. The method is extremely sensitive, and is economical in consumption of sample (10 mg), solvent (40–50 mL methanol), and time (2–2.5 h). The method was validated for specificity, precision, and accuracy in accordance with International Conference on Harmonization (ICH) guidelines on technical requirements for registration of pharmaceuticals for human use. The relative standard deviation of intra-day and inter-day analysis was in the range 0.56–1.33% and accuracy, as determined by measurement of recovery, was, on average, 98%. In addition, the effect of drug solvent ratio on the quantification procedure was also studied in detail. In comparison with other methods for analysis of andrographolide, this method has the advantage of minimizing consumption of expensive drug samples, organic solvents, and other resources.
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| Journal of Planar Chromatography - Modern TLC | |
|---|---|
| Language | English |
| Size | A4 |
| Year of Foundation |
1988 |
| Volumes per Year |
1 |
| Issues per Year |
6 |
| Founder | Akadémiai Kiadó |
| Founder's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. |
| Publisher | Akadémiai Kiadó Springer Nature Switzerland AG |
| Publisher's Address |
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245. CH-6330 Cham, Switzerland Gewerbestrasse 11. |
| Responsible Publisher |
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| ISSN | 0933-4173 (Print) |
| ISSN | 1789-0993 (Online) |
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