JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 23: Issue 2
Optimization of an HPTLC method for separation and identification of phenolic compounds
Authors:
Madan Pandey National Botanical Research Institute Pharmacognosy and Ethnopharmacology Division Lucknow 226 001 India

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Subha Rastogi National Botanical Research Institute Pharmacognosy and Ethnopharmacology Division Lucknow 226 001 India

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Ajay Rawat National Botanical Research Institute Pharmacognosy and Ethnopharmacology Division Lucknow 226 001 India

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Pages:
108–111
Online Publication Date:
03 May 2010
Publication Date:
01 Apr 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.23.2010.2.3
Keywords:
HPTLC; Phenolic compounds; Standardization
Restricted access

Phenolic compounds are plant metabolites found widely spread throughout the plant kingdom. Phenolic acids, low-molecular-weight phenolic compounds, are precursors of a variety of antimicrobial compounds, and are important in plant defense responses. They are also involved in the plants’ physiological activity, which depends on their antioxidant activity, i.e. the ability to scavenge both active oxygen species and electrophiles, their ability to inhibit nitrosation and to chelate metal ions, their potential for autooxidation, and their capability to modulate the activity of some cellular enzymes. Because of their importance in the standardization of plant products, an attempt has been made to develop a simple, rapid, and precise HPTLC method for the separation and identification of different phenolic compounds in a single analysis. A TLC method which separates, identifies, and quantifies six different phenolic compounds-gallic acid, ferulic acid, syringic acid, catechin, protocatechuic acid, and vanillin-with a single mobile phase was established. It was then validated for selectivity, linearity, precision, accuracy and robustness. The method was found to enable rapid, precise, accurate, simple, and cost-effective analysis of these important phenolic compounds for standardization of different plant materials and herbal products.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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