Phenolic compounds are plant metabolites found widely spread throughout the plant kingdom. Phenolic acids, low-molecular-weight phenolic compounds, are precursors of a variety of antimicrobial compounds, and are important in plant defense responses. They are also involved in the plants’ physiological activity, which depends on their antioxidant activity, i.e. the ability to scavenge both active oxygen species and electrophiles, their ability to inhibit nitrosation and to chelate metal ions, their potential for autooxidation, and their capability to modulate the activity of some cellular enzymes. Because of their importance in the standardization of plant products, an attempt has been made to develop a simple, rapid, and precise HPTLC method for the separation and identification of different phenolic compounds in a single analysis. A TLC method which separates, identifies, and quantifies six different phenolic compounds-gallic acid, ferulic acid, syringic acid, catechin, protocatechuic acid, and vanillin-with a single mobile phase was established. It was then validated for selectivity, linearity, precision, accuracy and robustness. The method was found to enable rapid, precise, accurate, simple, and cost-effective analysis of these important phenolic compounds for standardization of different plant materials and herbal products.
G. Seneviratne, H.S. Jayasinghearachchi
, Plant Soil