JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 23: Issue 2
Thin-layer chromatographic analysis of carotenoids in plant and animal samples
Authors:
Alam Zeb

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and
Michael Murkovic Graz University of Technology Institute for Biochemistry Graz Austria

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Pages:
94–103
Online Publication Date:
03 May 2010
Publication Date:
01 Apr 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.23.2010.2.1
Keywords:
Carotenoids; Analysis; Food; Thin-layer chromatography; Separation techniques
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Carotenoids are among the most widespread and important pigments in living organisms. They are found in common foods and vegetables. The characteristic pattern of alternating single and double bonds in the polyene backbone enables them to absorb excess energy from other molecules. The nature of the specific end groups on carotenoids may effect their polarity, thus solubility ranges from acetone to hexane. Because of this wide range of polarity, specific extraction and separation procedures are required. In these procedures use of planar chromatography in food analysis might seem a minor aspect of carotenoid analysis. This review describes available data on analysis of carotenoids by thin-layer chromatography (TLC). It has been found that petroleum ether, acetone, and hexane are the major mobile phases used for TLC. Thin-layer chromatography was found to have the potential to be the first choice for analysis of carotenoids in biological samples. The uses of other, orthogonal chromatographic methods, for example HPLC, spectroscopy (mass spectroscopy), scanning densitometry, and image analysis with TLC can enable precise analysis of carotenoids.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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