Direct bioautography performed with luminescence gene-tagged bacteria enables almost real-time detection of antimicrobial compounds in plant extracts. This method for the detection of chamomile (
) components with antibacterial effect against Bacillus subtilis soil bacteria was more sensitive than commonly used bioautographic visualization by staining with a tetrazolium salt. Some compounds had a strong inhibiting effect only via the bioluminescence measurement. Extraction of antibacterial components of chamomile flowers was most effective with 50% ethanol; slightly lower efficiency was achieved with acetone and methanol, and hexane was least effective. The results were confirmed by using luminescent
Pseudomonas syringae pv. maculicola
plant pathogen bacteria.
E. Tyihák, Á.M. Móricz, P.G. Ott
M. Waksmundzka-Hajnos, J. Sherma, T. Kowalska
(eds.), Thin-Layer Chromatography in Phytochemistry, CRC Press, Taylor & Frances Group, Boca Raton, 2008. pp. 193–213.
Ott P.G., '', in Thin-Layer Chromatography in Phytochemistry, (2008) -.
Ott P.G.Thin-Layer Chromatography in Phytochemistry2008)| false