JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 23: Issue 3
Usefulness of transgenic luminescent bacteria in direct bioautographic investigation of chamomile extracts
Authors: Ágnes Móricz 1 , Ernő Tyihák 1 , and Péter Ott 1
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  • 1 Hungarian Academy of Sciences Plant Protection Institute Herman O. u. 15 1022 Budapest Hungary
DOI:
https://doi.org/10.1556/jpc.23.2010.3.2
Page Count:
180–183
Publication Date:
01 Jun 2010
Online Publication Date:
17 Jun 2010
Article Category:
Research Article
Restricted access

Direct bioautography performed with luminescence gene-tagged bacteria enables almost real-time detection of antimicrobial compounds in plant extracts. This method for the detection of chamomile ( Matricaria recutita ) components with antibacterial effect against Bacillus subtilis soil bacteria was more sensitive than commonly used bioautographic visualization by staining with a tetrazolium salt. Some compounds had a strong inhibiting effect only via the bioluminescence measurement. Extraction of antibacterial components of chamomile flowers was most effective with 50% ethanol; slightly lower efficiency was achieved with acetone and methanol, and hexane was least effective. The results were confirmed by using luminescent Pseudomonas syringae pv. maculicola plant pathogen bacteria.

  • D.E. Wedge, D.G. Nagle , J. Nat. Prod. 63 (2000) 1050–1054.

    Nagle D.G. , '' (2000 ) 63 J. Nat. Prod. : 1050 -1054.

  • K. Hostettmann , Pure App. Chem. 70 (1999) 1109–1113.

    Hostettmann K. , '' (1999 ) 70 Pure App. Chem. : 1109 -1113.

  • E. Tyihák, Á.M. Móricz, P.G. Ott , in: M. Waksmundzka-Hajnos, J. Sherma, T. Kowalska (eds.), Thin-Layer Chromatography in Phytochemistry, CRC Press, Taylor & Frances Group, Boca Raton, 2008. pp. 193–213.

    Ott P.G. , '', in Thin-Layer Chromatography in Phytochemistry , (2008 ) -.

  • R.P. Stowe, D.W. Koenig, S.K. Mishra, D.L. Pierson , J. Microbiol. Meth. 22 (1995) 283–292.

    Pierson D.L. , '' (1995 ) 22 J. Microbiol. Meth. : 283 -292.

  • S.A.B. Jabber, P.R. Twentyman, J.V. Watson , Br. J. Cancer 60 (1989) 523–528.

    Watson J.V. , '' (1989 ) 60 Br. J. Cancer : 523 -528.

  • M.C. Pagliacci, F. Spinozzi, G. Migliorati, G. Fumi, M. Smacchia, F. Grignani, C. Riccardi, I. Nicoletti , Eur. J. Cancer 29A (1993) 1573–1577.

    Nicoletti I. , '' (1993 ) 29A Eur. J. Cancer : 1573 -1577.

  • D. Bernharda, W. Schwaiger, R. Crazzolara, I. Tinhofer, R. Kofler, A. Csordas , Cancer Lett. 195 (2003) 193–199.

    Csordas A. , '' (2003 ) 195 Cancer Lett. : 193 -199.

  • C. Weins, H. Jork , J. Chromatogr. A 750 (1996) 403–407.

    Jork H. , '' (1996 ) A750 J. Chromatogr : 403 -407.

  • G. Eberz, H.G. Rast, K. Burger, W. Kreiss, C. Weisemann , Chromatographia 43 (1996) 5–9.

    Weisemann C. , '' (1996 ) 43 Chromatographia : 5 -9.

  • S.M. Verbitski, G.T. Gourdin, L.M. Ikenouye, J.D. McChesney , Am. Biotechnol. Lab. 24 (2006) 40–42.

    McChesney J.D. , '' (2006 ) 24 Am. Biotechnol. Lab. : 40 -42.

  • W. Schulz, W. Seitz, S.C. Weiss, W.H. Weber, M. Böhm, D. Flottmann , J. Planar Chromatogr. 21 (2008) 427–430.

    Flottmann D. , '' (2008 ) 21 J. Planar Chromatogr. : 427 -430.

  • F. Dane, J.J. Shaw , J. Appl. Bact. 80 (1996) 73–80.

    Shaw J.J. , '' (1996 ) 80 J. Appl. Bact. : 73 -80.

  • J. Fan, C. Crooks, C. Lamb , Plant J. 53 (2008) 393–399.

    Lamb C. , '' (2008 ) 53 Plant J. : 393 -399.

  • P. Sanz, L.D. Teel, F. Alem, H.M. Carvalho, S.C. Darnell, A.D. O’Brien , Infect. Immun. 76 (2008) 1036–1047.

    O’Brien A.D. , '' (2008 ) 76 Infect. Immun. : 1036 -1047.

  • E. Tyihák, E. Mincsovics, Gy. Kátay, Zs. Király-Véghely, Á.M. Móricz, P.G. Ott , J. Planar Chromatogr. 21 (2008) 15–20.

    Ott P.G. , '' (2008 ) 21 J. Planar Chromatogr. : 15 -20.

  • P.Z. Wang, R.H. Doi , J. Biol. Chem. 259 (1984) 8619–8625.

    Doi R.H. , '' (1984 ) 259 J. Biol. Chem. : 8619 -8625.

  • C. Anagnostopoulos, J. Spizizen , J. Bacteriol. 81 (1961) 741–746.

    Spizizen J. , '' (1961 ) 81 J. Bacteriol. : 741 -746.

  • E.O. King, M.K. Ward, D.E. Raney , J. Lab. Clin. Med. 44 (1954)
Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993
Keywords:
Bioautography; Chamomile ( Matricaria recutita ); Bioluminescence; Pseudomonas syringae ; Bacillus subtilis

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