JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 23: Issue 6
OPLC of carbohydrate content for quality control of commercial Romanian wines
Authors:
Mihaela Vlassa Babeş-Bolyai University Raluca Ripan Institute for Research in Chemistry 30 Fantanele Street 400294 Cluj-Napoca Romania

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Virginia Coman Babeş-Bolyai University Raluca Ripan Institute for Research in Chemistry 30 Fantanele Street 400294 Cluj-Napoca Romania

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Miuţa Filip Babeş-Bolyai University Raluca Ripan Institute for Research in Chemistry 30 Fantanele Street 400294 Cluj-Napoca Romania

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Florina Oniţă Babeş-Bolyai University Raluca Ripan Institute for Research in Chemistry 30 Fantanele Street 400294 Cluj-Napoca Romania

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Pages:
400–405
Online Publication Date:
09 Dec 2010
Publication Date:
01 Dec 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.23.2010.6.3
Keywords:
Sucrose; Glucose; Fructose; OPLC; Wine type
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Arapid and simple method for simultaneous quantitative analysis of the natural carbohydrates fructose, glucose, and sucrose in different types of wine (white and red; dry, semi-dry, semi-sweet, and sweet) by OPLC in overrunning mode is presented. The compounds were separated on precoated silica gel F254 OPLC plates with acetonitrile-water 26:4 (v/v) as mobile phase. Visualization was by spraying with a mixed solution of aniline and diphenylamine, then densitometry in absorbance mode was performed at 420 nm. Linear regression analysis of the calibration data was indicative of a good linear relationship between the peak area and the concentration in the range 0.5–5.0 μg per band (rglucose 0.99051, rfructose 0.98547, rsucrose 0.99506). In this paper we give the results obtained from analysis of 49 samples of white and red wines. In the most of the samples we did not find sucrose, only fructose and glucose in different ratios depending on the type of the wine. We found few white and red wine samples for which the content of reducing sugars was not in agreement with the label description. The results obtained for the wines show this OPLC method can be useful for routine monitoring of the quality of commercial wine. Amajor advantage of the method is that it enables simultaneous analysis of approximately 20 samples in less than 2 h.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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