JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 23: Issue 6
Investigation of thyme (Thymus vulgaris L.) essential oil by use of the BioArena system
Authors:
Ágnes Móricz Hungarian Academy of Sciences Plant Protection Institute Herman O. Str. 15 1022 Budapest Hungary

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Györgyi Horváth University of Pécs Institute of Pharmacognosy, Medical School Rókus Str. 2 7624 Pécs Hungary

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Péter Molnár University of Pécs Institute of Pharmacognosy, Medical School Rókus Str. 2 7624 Pécs Hungary

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Béla Kocsis University of Pécs Institute of Medical Microbiology and Immunology, Medical School Szigeti Str. 12 7624 Pécs Hungary

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Andrea Böszörményi Semmelweis University Institute of Pharmacognosy, Faculty of Pharmacy Üllői Str. 26 1085 Budapest Hungary

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Éva Lemberkovics Semmelweis University Institute of Pharmacognosy, Faculty of Pharmacy Üllői Str. 26 1085 Budapest Hungary

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Péter Ott Hungarian Academy of Sciences Plant Protection Institute Herman O. Str. 15 1022 Budapest Hungary

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Pages:
406–410
Online Publication Date:
09 Dec 2010
Publication Date:
01 Dec 2010
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.23.2010.6.4
Keywords:
Bioautography; BioArena; Formaldehyde; Thymus vulgaris L.; Pseudomonas syringae ; Bacillus subtilis
Restricted access

The composition of the essential oil of Thymus vulgaris L. has been determined by GC-FID and GC-MS. Because separation of thymol, carvacrol, and linalool, components of the essential oil, was more efficient by overpressured layer chromatography (OPLC) than by conventional thin-layer chromatography (TLC), the forced flow technique was used before biological detection. All three test compounds had antibacterial effect against the phytopathogenic bacterium Pseudomonas syringae pv. maculicola, in bioautography, although in essential oil thymol was present in sufficient quantity to produce an inhibiting zone in the adsorbent layer. In BioArena investigations, when reduced glutathione as a formaldehyde (HCHO) capturer was dissolved in the cell suspension before bioautographic exposure to the essential oil, the characteristic inhibiting activity of thymol and carvacrol against Bacillus subtilis soil bacteria was reduced, whereas the presence of the HCHO precursors NGmonomethyl-l-arginine or Nɛ-monomethyl-l-lysine enhanced their antibacterial effect. These results suggest that HCHO and its reaction products may be involved in the antibacterial activity of thymol and carvacrol.

  • A. Zarzuelo, E. Crespo, in: Thyme, the genus Thymus, E. Stahl-Biskup, F. Sáez (eds.), Taylor and Francis, London-New York, 2002, pp. 263–292.

    Crespo E. , '', in Thyme, the genus Thymus , (2002 ) -.

  • J. Barnes, L.A. Anderson, J.D. Phillipson, Herbal Medicines, Pharmaceutical Press, London-Chicago, 2002, pp. 462–464.

    Phillipson J.D. , '', in Herbal Medicines , (2002 ) -.

  • C. Rota, J.J. Carraminana, J. Burillo, A. Herrera, J. Food Protect. 67 (2004) 1252–1256.

    Herrera A. , '' (2004 ) 67 J. Food Protect. : 1252 -1256 .

  • N. Tabanca, B. Demirci, K. Can Baser, E. Mincsovics, S.I. Khan, M.R. Jacob, D.E. Wedge, J. Chromatogr. B 850 (2007) 221–229.

    Wedge D.E. , '' (2007 ) 850 J. Chromatogr. B : 221 -229 .

  • K.A. Hammer, C.F. Carson, T.V. Riley, Simply Essential 36 (2000) 18–22.

    Riley T.V. , '' (2000 ) 36 Simply Essential : 18 -22 .

  • M. Chamberlain, Ann. Inst. Pasteur 1 (1887) 153–164

    Chamberlain M. , '' (1887 ) 1 Ann. Inst. Pasteur : 153 -164 .

  • C. Rota, J.J. Carraminana, J. Burillo, A. Herrera, J. Food Protect. 67 (2004) 1252–1256.

    Herrera A. , '' (2004 ) 67 J. Food Protect. : 1252 -1256 .

  • R.R. Nelson, J. Antimicrob. Chemother. 40 (1997) 305–306.

    Nelson R.R. , '' (1997 ) 40 J. Antimicrob. Chemother. : 305 -306 .

  • Gy. Horváth, N. Jámbor, A. Végh, A. Böszörményi, É. Lemberkovics, É. Héthelyi, K. Kovács, B. Kocsis, Flavour Fragr. J. 25 (2010) 178–182.

    Kocsis B. , '' (2010 ) 25 Flavour Fragr. J. : 178 -182 .

  • B.J. Juven, J. Kanner, F. Schued, H. Weisslowicz, J. Appl. Bacteriol. 76 (1994) 626–631.

    Weisslowicz H. , '' (1994 ) 76 J. Appl. Bacteriol. : 626 -631 .

  • S.E. Megalla, N.E.M. El-Keltawi, S.A. Ross, Herba Pol. 26 (1980) 181–186.

    Ross S.A. , '' (1980 ) 26 Herba Pol. : 181 -186 .

  • C. Lens-Lisbonne, A. Cremieux, C. Maillard, G. Balansard, J. Pharm. Belg. 42 (1987) 297–302.

    Balansard G. , '' (1987 ) 42 J. Pharm. Belg. : 297 -302 .

  • L. Botz, S. Nagy, B. Kocsis, in: Sz. Nyiredy (ed.), Planar Chromatography — A Retrospective View for the Third Millennium, Springer, Budapest, 2001, pp. 489–51.

    Kocsis B. , '', in Planar Chromatography — A Retrospective View for the Third Millennium , (2001 ) -.

  • W.J. Begue, R.M. Kline, J. Chromatogr. 64 (1972) 182–184.

    Kline R.M. , '' (1972 ) 64 J. Chromatogr. : 182 -184 .

  • M.O. Hamburger, G.A. Cordell, J. Nat. Prod. 50 (1987) 19–22.

    Cordell G.A. , '' (1987 ) 50 J. Nat. Prod. : 19 -22 .

  • C. Weins, H. Jork, J. Chromatogr. A 750 (1996) 403–407.

    Jork H. , '' (1996 ) 750 J. Chromatogr. A : 403 -407 .

  • G. Eberz, H.G. Rast, K. Burger, W. Kreiss, C. Weisemann, Chromatographia 43 (1996) 5–9.

    Weisemann C. , '' (1996 ) 43 Chromatographia : 5 -9 .

  • S.M. Verbitski, G.T. Gourdin, L.M. Ikenouye, J.D. McChesney, Am. Biotechnol. Lab. 24 (2006) 40–42.

    McChesney J.D. , '' (2006 ) 24 Am. Biotechnol. Lab. : 40 -42 .

  • Á.M. Móricz, E. Tyihák, P.G. Ott, J. Planar Chromatogr. 23 (2010) 180–183.

    Ott P.G. , '' (2010 ) 23 J. Planar Chromatogr. : 180 -183 .

  • E. Tyihák, Á.M. Móricz, P.G. Ott, in: M. Waksmundzka-Hajnos, J. Sherma, T. Kowalska (eds.), Thin-layer chromatography in phytochemistry, CRC Press, Boca Raton, 2008, pp. 193–213.

    Ott P.G. , '', in Thin-layer chromatography in phytochemistry , (2008 ) -.

  • Á.M. Móricz, N. Adányi, E. Horváth, P.G. Ott, E. Tyihák, J. Planar Chromatogr. 21 (2008) 417–422.

    Tyihák E. , '' (2008 ) 21 J. Planar Chromatogr. : 417 -422 .

  • E. Tyihák, E. Mincsovics, Gy. Kátay, Zs. Király-Véghely, Á.M. Móricz, P.G. Ott, J. Planar Chromatogr. 21 (2008) 15–20.

    Ott P.G. , '' (2008 ) 21 J. Planar Chromatogr. : 15 -20 .

  • E. Mincsovics, M. Garami, L. Kecskés, B. Tapa, Z. Végh, Gy. Kátay, E. Tyihák, J. AOAC Int. 82 (1999) 587–598.

    Tyihák E. , '' (1999 ) 82 J. AOAC Int. : 587 -598 .

  • E. Tyihák, E. Mincsovics, in: Sz. Nyiredy (ed.), Planar Chromatography — A Retrospective View for the Third Millennium, Springer, Budapest, 2001, pp. 137–176.

    Mincsovics E. , '', in Planar Chromatography — A Retrospective View for the Third Millennium , (2001 ) -.

  • Gy. Horváth, L.Gy. Szabó, É. Lemberkovics, L. Botz, B. Kocsis, J. Planar Chromatogr. 17 (2004) 300–304.

    Kocsis B. , '' (2004 ) 17 J. Planar Chromatogr. : 300 -304 .

  • E. Mincsovics, N. Tabanca, Á.M. Móricz, D.E. Wedge, E. Tyihák, J. Planar Chromatogr. 23 (2010) 180–183.

    Tyihák E. , '' (2010 ) 23 J. Planar Chromatogr. : 180 -183 .

  • A. Csiba, L. Trézl, E. Tyihák, H. Graber, E. Vári, G. Téglás, I. Rusznák, Acta Physiol. Acad. Sci. Hung. 59 (1982) 35–43.

    Rusznák I. , '' (1982 ) 59 Acta Physiol. Acad. Sci. Hung. : 35 -43 .

  • E. Tyihák, Á. Móricz, P.G. Ott, in: M.A. Cser et al. (eds.), Metal Ions in Biology and Medicine, vol. 8, John Libbey, Eurotext, Paris, 2004.

    Ott P.G. , '', in Metal Ions in Biology and Medicine , (2004 ) -.

  • European Pharmacopoeia, 4th edition, Druckerei C.H. Beck, Nördlingen, 2002, p. 183.

  • P.Z. Wang, R.H. Doi, J. Biol. Chem. 259 (1984) 8619–8625.

    Doi R.H. , '' (1984 ) 259 J. Biol. Chem. : 8619 -8625 .

  • J. Fan, C. Crooks, C. Lamb, Plant J. 53 (2008) 393–399.

    Lamb C. , '' (2008 ) 53 Plant J. : 393 -399 .

  • E.O. King, M.K. Ward, D.E. Raney, J. Lab. Clin. Med. 44 (1954) 301–317.

    Raney D.E. , '' (1954 ) 44 J. Lab. Clin. Med. : 301 -317 .

  • C. Anagnostopoulos, J. Spizizen, J. Bacteriol. 81 (1961) 741–746.

    Spizizen J. , '' (1961 ) 81 J. Bacteriol. : 741 -746 .

  • S. Huszti, E. Tyihák, FEBS Lett. 209 (1986) 362–366.

    Tyihák E. , '' (1986 ) 209 FEBS Lett. : 362 -366 .

  • L. Trézl, I. Rusznák, E. Tyihák, T. Szarvas, B. Szende, Biochem. J. 214 (1983) 289–292.

    Szende B. , '' (1983 ) 214 Biochem. J. : 289 -292 .

  • L. Trézl, L. Hullán, Z.M. Jászay, T. Szarvas, I. Petneházy, B. Szende, J. Bocsi, Z. Takáts, K. Vékey, L. Tőke, Mol. Cell. Biochem. 244 (2003) 167–176.

    Tőke L. , '' (2003 ) 244 Mol. Cell. Biochem. : 167 -176 .

  • Y.J. Shi, F. Lan, C. Matson, P. Mulligan, J.R. Whetstine, P.A. Cole, R.A. Casero, Y. Shi, Cell 119 (2004) 941–953.

    Shi Y. , '' (2004 ) 119 Cell : 941 -953 .

  • L. Trézl, J. Pipek, J. Mol. Struct. (Theochem.) 170 (1988) 213–223.

    Pipek J. , '' (1988 ) 170 J. Mol. Struct. (Theochem.) : 213 -223 .

  • P. Wentworth, J.E. McDunn, A.D. Wentworth, C. Takeuchi, J. Nieva, T. Jones, C. Bautista, J.M. Ruedi, A. Gutierrez, K.D. Janda, B.M. Babior, A. Eschenmoser, R.A. Lerner, Science 298 (2002) 2195–2199.

    Lerner R.A. , '' (2002 ) 298 Science : 2195 -2199 .

  • P. Wentworth Jr, A.D. Wentworth, X.Y. Zhu, I.A. Wilson, K.D. Janda, A. Eschenmoser, R.A. Lerner, Proc. Natl. Acad. Sci. USA 100 (2003) 1490–1493.

    Lerner R.A. , '' (2003 ) 100 Proc. Natl. Acad. Sci. USA : 1490 -1493 .
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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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