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  • 1 Hungarian Academy of Sciences Plant Protection Institute Herman O. Str. 15 1022 Budapest Hungary
  • 2 University of Pécs Institute of Pharmacognosy, Medical School Rókus Str. 2 7624 Pécs Hungary
  • 3 University of Pécs Institute of Medical Microbiology and Immunology, Medical School Szigeti Str. 12 7624 Pécs Hungary
  • 4 Semmelweis University Institute of Pharmacognosy, Faculty of Pharmacy Üllői Str. 26 1085 Budapest Hungary
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The composition of the essential oil of Thymus vulgaris L. has been determined by GC-FID and GC-MS. Because separation of thymol, carvacrol, and linalool, components of the essential oil, was more efficient by overpressured layer chromatography (OPLC) than by conventional thin-layer chromatography (TLC), the forced flow technique was used before biological detection. All three test compounds had antibacterial effect against the phytopathogenic bacterium Pseudomonas syringae pv. maculicola, in bioautography, although in essential oil thymol was present in sufficient quantity to produce an inhibiting zone in the adsorbent layer. In BioArena investigations, when reduced glutathione as a formaldehyde (HCHO) capturer was dissolved in the cell suspension before bioautographic exposure to the essential oil, the characteristic inhibiting activity of thymol and carvacrol against Bacillus subtilis soil bacteria was reduced, whereas the presence of the HCHO precursors NGmonomethyl-l-arginine or N ɛ-monomethyl-l-lysine enhanced their antibacterial effect. These results suggest that HCHO and its reaction products may be involved in the antibacterial activity of thymol and carvacrol.

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