JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 24: Issue 1
Simultaneous identification and quantitative analysis of eight cephalosporins in pharmaceutical formulations by TLC-densitometry
Authors:
Monika Dąbrowska Jagiellonian University, Collegium Medicum Department of Inorganic and Analytical Chemistry Medyczna 9 Kraków Poland

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Małgorzata Starek Jagiellonian University, Collegium Medicum Department of Inorganic and Analytical Chemistry Medyczna 9 Kraków Poland

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Sabina Pikulska Jagiellonian University, Collegium Medicum Department of Inorganic and Analytical Chemistry Medyczna 9 Kraków Poland

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Pages:
23–29
Online Publication Date:
15 Feb 2011
Publication Date:
01 Feb 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.24.2011.1.4
Keywords:
Thin-layer chromatography; Densitometric detection; Cephalexin; Cefadroxil; Cefazolin; Cefaclor; Cefuroxime; Cefuroxime axetil; Ceftriaxone; Cefotaxime
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A rapid, accurate, and sensitive densitometric TLC method has been established and validated for simultaneous analysis of eight β-lactam antibiotics (cephalexin, cefadroxil, and cefazolin (first-generation), cefaclor, cefuroxime, and cefuroxime axetil (second-generation), and ceftriaxone and cefotaxime (third-generation)). Chromatographic separation was achieved on silica gel F254 plates with chloroform-ethyl acetate-glacial acetic acid-water 4:4:4:1 (v/v) as mobile phase. Densitometric detection was performed at 275 nm. Statistical evaluation showed the performance of the method was satisfactory. Accuracy was in the range 98.30–100.85% and precision, as RSD, was from 0.4 to 2.45%.

  • Z. Ochocki, A. Stańzak, Farm. Pol. 61 (2005) 171–182.

    Stańzak A. , '' (2005 ) 61 Farm. Pol. : 171 -182 .

  • H.P. Rang, M.M. Dale, J.M. Ritter, Farmakologia kliniczna, Wyd. Czelej, Lublin, 2001.

    Ritter J.M. , '', in Farmakologia kliniczna , (2001 ) -.

  • A. Zejc, M. Gorczyca, Chemia Leków, PZWL, Warszawa, 2002.

    Gorczyca M. , '', in Chemia Leków , (2002 ) -.

  • M. Zając, E. Pawełczyk, Chemia Leków, Wyd. AM, Poznań, 2000.

  • D. Pitkin, P. Actor, W. Holl, J.A. Weisbach, Antimicrob. Agents Chemother. 5 (1974) 223–227.

    Weisbach J.A. , '' (1974 ) 5 Antimicrob. Agents Chemother. : 223 -227 .

  • Y.H. Khang, H. Shankar, F. Senatore, Biotechnol. Tech. 2 (1988) 57–62.

    Senatore F. , '' (1988 ) 2 Biotechnol. Tech. : 57 -62 .

  • R. Denooz, C. Charlier, J. Chromatogr. B 864 (2008) 161–167.

    Charlier C. , '' (2008 ) 864 J. Chromatogr. B : 161 -167 .

  • V.F. Samanidou, E.A. Hapeshi, I.N. Papadoyannis, J. Chromatogr. B 788 (2003) 147–158.

    Papadoyannis I.N. , '' (2003 ) 788 J. Chromatogr. B : 147 -158 .

  • F. Pehoureq, C. Jarry, J. Chromatogr. A 812 (1998) 159–178.

    Jarry C. , '' (1998 ) 812 J. Chromatogr. A : 159 -178 .

  • R.V. Oliveira, Q.B. Cass, J. Agric. Food Chem. 54 (2006) 1180–1187.

    Cass Q.B. , '' (2006 ) 54 J. Agric. Food Chem. : 1180 -1187 .

  • V.F. Samanidou, A.S. Ioannou, I.N. Papadoyannis, J. Chromatogr. B 809 (2004) 175–182.

    Papadoyannis I.N. , '' (2004 ) 809 J. Chromatogr. B : 175 -182 .

  • L.K. Sorensen, L. Snor, J. Chromatogr. A 882 (2000) 145–151.

    Snor L. , '' (2000 ) 882 J. Chromatogr. A : 145 -151 .

  • M.A. Omar, O.H. Abdelmageed, T.Z. Attia, Int. J. Anal. Chem. 3 (2009) 1–12.

    Attia T.Z. , '' (2009 ) 3 Int. J. Anal. Chem. : 1 -12 .

  • N.N. Okoye, G.I.C. Nwokedi, N.N. Ukwueze, F.B.C. Okoye, Sci. Res. Essay 2 (2007) 342–347.

    Okoye F.B.C. , '' (2007 ) 2 Sci. Res. Essay : 342 -347 .

  • M.A. Omar, O.H. Abdelmageed, T.Z. Attia, Talanta 77 (2009) 1394–404.

    Attia T.Z. , '' (2009 ) 77 Talanta : 1394 -404 .

  • L. Niu, Z. Song, X. He, Drug. Metab. Lett. 8 (2009) 144–151.

    He X. , '' (2009 ) 8 Drug. Metab. Lett. : 144 -151 .

  • A.R. Solangi, S.Q. Memon, M.Y. Khuhawar, M. Bhanger, Acta Chromatogr. 19 (2007) 81–96.

    Bhanger M. , '' (2007 ) 19 Acta Chromatogr. : 81 -96 .

  • S.C. Dhansenar, J. Planar Chromatogr. 12 (1999) 114–119.

    Dhansenar S.C. , '' (1999 ) 12 J. Planar Chromatogr. : 114 -119 .

  • I.M. Choma, J. Liq. Chromatogr. Relat. Technol. 30 (2007) 2231–2244.

    Choma I.M. , '' (2007 ) 30 J. Liq. Chromatogr. Relat. Technol. : 2231 -2244 .

  • T. Tuzimski, J. Planar Chromatogr. 17 (2004) 46–50.

    Tuzimski T. , '' (2004 ) 17 J. Planar Chromatogr. : 46 -50 .

  • S.A. Nabi, E. Laiq, A. Islam, Acta Chromatogr. 14 (2004) 92–101.

    Islam A. , '' (2004 ) 14 Acta Chromatogr. : 92 -101 .

  • G. Misztal, A. Szalast, H. Hopkała, Chem. Anal. 43 (1998) 357–363.

    Hopkała H. , '' (1998 ) 43 Chem. Anal. : 357 -363 .

  • R. Bhushan, G.T. Thiong’o, Biomed. Chromatogr. 16 (2002) 165–174.

    Thiong’o G.T. , '' (2002 ) 16 Biomed. Chromatogr. : 165 -174 .

  • S. Eric, D. Agbaba, S. Vladimirov, D. Zivanov-Stakic, J. Planar Chromatogr. 13 (2000) 88–92.

    Zivanov-Stakic D. , '' (2000 ) 13 J. Planar Chromatogr. : 88 -92 .

  • R. Bhushan, V. Parshad, Biomed. Chromatogr. 10 (1996) 258–260.

    Parshad V. , '' (1996 ) 10 Biomed. Chromatogr. : 258 -260 .

  • G. Misztal, J. Liq. Chromatogr. Relat. Technol. 22 (1999) 1589–1598.

    Misztal G. , '' (1999 ) 22 J. Liq. Chromatogr. Relat. Technol. : 1589 -1598 .

  • S. Eric-Jovanovic, D. Agbaba, D. Zivanov-Stakic, S. Vladimirov, J. Pharm. Biomed. Anal. 18 (1998) 893–898.

    Vladimirov S. , '' (1998 ) 18 J. Pharm. Biomed. Anal. : 893 -898 .

  • D. Agbaba, S. Eric, D. Zivanov-Stakic, S. Vladimirov, Biomed. Chromatogr. 12 (1998) 133–135.

    Vladimirov S. , '' (1998 ) 12 Biomed. Chromatogr. : 133 -135 .

  • J. Krzek, M. D1browska-Tylka, Chromatographia 58 (2003) 231–234.

    D1browska-Tylka M. , '' (2003 ) 58 Chromatographia : 231 -234 .

  • G. Saleh, H. Askal, N. Omar, Anal. Lett. 23 (1990) 833–841.

    Omar N. , '' (1990 ) 23 Anal. Lett. : 833 -841 .

  • J. Pawlaczyk, M. Zając, Walidacja metod analizy chemicznej, Wyd. AM, Poznań, 2001.

    Zając M. , '', in Walidacja metod analizy chemicznej , (2001 ) -.

  • ICH Guideline Q2 (R1) on “Validation of Analytical Procedures: Text and Methodology”, International Conference on Harmonization, Geneva (6 Nov 1996, incorp. Nov 2005).

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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