Authors:
Saleh AlqasoumiAl Kharj University Department of Pharmacognosy, College of Pharmacy Al Kharj Kingdom of Saudi Arabia

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Prawez AlamAl Kharj University Department of Pharmacognosy, College of Pharmacy Al Kharj Kingdom of Saudi Arabia

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Adnan Al-RehailyKing Saud University Department of Pharmacognosy, College of Pharmacy Riyadh Kingdom of Saudi Arabia

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Faiyaz ShakeelAl-Arab Medical University Department of Pharmaceutics, Faculty of Pharmacy Benghazi Libya

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Maged Abdel-KaderAl Kharj University Department of Pharmacognosy, College of Pharmacy Al Kharj Kingdom of Saudi Arabia

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A densitometric high-performance thin-layer chromatographic (HPTLC) method for analysis of hydroquinone has been developed and validated. Chromatography was performed on aluminum foilbacked silica gel 60 F254 plates with chloroform-methanol 85:15 (% v/v) as mobile phase. This system furnished a compact band for hydroquinone at RF 0.51. Hydroquinone was quantified densitometrically at 289 nm. The limits of detection (LOD) and quantification (LOQ) were 38.50 and 115.50 ng per band, respectively. High precision and accuracy were achieved. The method was used for both qualitative and quantitative analysis of hydroquinone in commercial formulations. Because the method can effectively separate the hydroquinone in the presence of its degradation products, it can be used as a stability-indicating method.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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