JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 24: Issue 1
Development and validation of TLC method for analysis of Stresroak premix
Authors:
Kotagiri Ravikanth Ayurvet Limited R & D Center Village Katha P.O. Baddi-173205 District Solan Himachal Pradesh India

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Brijpal Singh Ayurvet Limited R & D Center Village Katha P.O. Baddi-173205 District Solan Himachal Pradesh India

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Ashish Gupta Ayurvet Limited R & D Center Village Katha P.O. Baddi-173205 District Solan Himachal Pradesh India

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Amit Singh Ayurvet Limited R & D Center Village Katha P.O. Baddi-173205 District Solan Himachal Pradesh India

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Anirudh Sharma Ayurvet Limited R & D Center Village Katha P.O. Baddi-173205 District Solan Himachal Pradesh India

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Abhishek Kumar Ayurvet Limited R & D Center Village Katha P.O. Baddi-173205 District Solan Himachal Pradesh India

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Pages:
66–71
Online Publication Date:
15 Feb 2011
Publication Date:
01 Feb 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.24.2011.1.13
Keywords:
HPTLC; Poultry; Stresroak premix; Gallic acid; Mangiferin; Withanolide A; Polyherbal
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With the realization of the growing popularity and demand for phytopharmaceuticals, standardization is becoming a mandatory part of the regulation of the herbal drug industry. This is applicable both to humans and veterinary formulations. Stresroak premix is a renowned polyherbal formulation for poultry. It is used as an immunomodulator, an antistress treatment, an adaptogenic, and a performance enhancer. It is a blend of Phyllanthus emblica L. (Euphorbiaceae), Withania somnifera L. (Solanacaeae), Mangifera indica L. (Anacardiaceae), Ocimum sanctum L. (Lamiaceae), and Shilajit. An HPTLC method has been established for standardization and quantification of gallic acid, mangiferin, and withanolide A in Stresroak premix. The average content of these markers in different batches of the formulation was 0.654, 0.627, and 0.325% (w/w), respectively. The method was validated for instrumental precision, repeatability, and accuracy. Average recovery of gallic acid, mangiferin, and withanolide Awas 100.41, 99.29, and 98.04% (w/w), respectively. The method is simple, precise, specific, and accurate and has potential for use in the routine quality control of the formulation and its raw materials.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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