JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 24: Issue 1
Densitometric HPTLC method for simultaneous quantification of sennosides A and B and gallic acid in a pharmaceutical dosage form
Authors:
Sachin Navale MIDC Ranjangaon Tulip Lab Pvt. Ltd, F-20/21 Pune 412220 India

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Vinod Kuber MIDC Ranjangaon Tulip Lab Pvt. Ltd, F-20/21 Pune 412220 India

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, and
Shrinivas Bhope MIDC Ranjangaon Tulip Lab Pvt. Ltd, F-20/21 Pune 412220 India

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Pages:
72–76
Online Publication Date:
15 Feb 2011
Publication Date:
01 Feb 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.24.2011.1.14
Keywords:
HPTLC; Sennoside A; Sennoside B; Gallic acid; Quantitation; Validation
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A densitometric HPTLC method has been established for simultaneous quantification of sennosides A and B and gallic acid in a laxative polyherbal pharmaceutical dosage form. Aluminum plates coated with silica gel 60 F254 as stationary phase were used with toluene-ethyl acetate-formic acid-methanol 8:8:4:5 (v/v) as mobile phase. Densitometric analysis was performed at 270 nm. Amounts of sennosides A and B and gallic acid were 10.48, 9.03, and 2.96% (w/w) respectively. The method was validated for specificity, linearity, precision, repeatability, and accuracy. Calibration plots were linear in the concentration range 114.0–427.5 ng per band for sennosides A and B and 100–375 ng per band for gallic acid. The correlation coefficients were 0.995, 0.998, and 0.997 for sennosides A and B and gallic acid, respectively. Relative standard deviation (RSD, [%]) for instrumental precision and repeatability of the method was 0.74, 0.49, 0.43 and 1.11, 1.08, 0.89 for sennosides A and B and gallic acid, respectively. Recovery was 96.28–97.22% for sennoside A, 98.06–100.84% for sennoside B, and 97.09–98.07% for gallic acid. The method is simple, precise, specific, accurate, and economical. It can be used for routine analysis of formulations containing Senna (Cassia angustifolia) and Haritaki (Terminalia chebula) extracts.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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