JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 24: Issue 2
Densitometric HPTLC analysis of kurarinone and sophoraflavanone G in Sophora flavescens root
Author:
Daniil Olennikov Russian Academy of Sciences Laboratory of Medical and Biological Research, Department of Biologically Active Substances, Institute of General and Experimental Biology, Siberian Division Sakh’yanovoy St. 6 670047 Ulan-Ude Russian Federation

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Pages:
121–124
Online Publication Date:
18 Apr 2011
Publication Date:
01 Apr 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.24.2011.2.7
Keywords:
Sophora flavescens ; Kurarinone; Sophoraflavanone G; HPTLC
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A thin-layer chromatographic method has been established for quantification of kurarinone and sophoraflavanone G in the roots of Sophora flavescens Soland. (Fabaceae). Chromatographic separation was performed on silica gel HPTLC plates with chloroform-methanol 10:1 (ν/ν) as mobile phase. The plates were scanned densitometrically at 285 nm. The method was validated for precision, repeatability, and accuracy. It was found to be precise — intra-day and inter-day RSD were 1.97–2.04% and 2.15–2.40%, respectively. Instrument precision and repeatability of the method were 0.43–0.56 and 1.81–1.84 (CV [%]), respectively. Accuracy was checked by measuring recovery at four levels; average recovery was 96.3–103.4% for kurarinone and 96.8–102.9% for sophoraflavanone G. The method was used for analysis of kurarinone and sophoraflavanone G in Sophora flavescens roots and was confirmed to be suitable for this purpose.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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