We report a comparative study of Juniperus virginiana and Juniperus communis essential oils (obtained by hydrodistillation of plant parts from the west of Romania or obtained commercially in Europe) by thin layer chromatographic and gas chromatographic-mass spectrometric analysis. Concentrations of cineole and guaiazulene, key compounds with insect repellent and anti-inflammatory activity, were evaluated. Monoterpenes were more abundant in essential oils from berries (40.2% in J. virginiana essential oil and 34.4% in J. communis essential oil), followed by sesquiterpene alcohols, but the latter were more abundant in essential oils from leaves (26.9% in J. virginiana and 16.9% in J. communis). We also discovered substantial amounts of monoterpene alcohols (8.9–9.5% and 6.4–11.6% for J. virginiana and J. communis, respectively) and sesquiterpenes (more abundant in J. communis essential oils: 21.1% in berries and 12.4% in leaves; in J. virginiana essential oils amounts of sesquiterpenes were only 5.9–6.8%). The key compounds cineole and guaiazulene were present at low absolute concentrations. The first was more abundant in J. communis essential oils (2.6–3.5 μg mL−1); guaiazulene was identified in J. virginiana essential oil only (0.7 μg mL−1 in essential oil from the leaves). Thinlayer chromatographic analysis of Juniperus essential oils revealed the importance of the monoterpenes, sesquiterpenes, and their corresponding alcohols in these mixtures. Cineole and guaiazulene spots from essential oils from autochthonous J. virginiana berries and leaves were very small, and were obscured by more abundant mono and sesquiterpenes (for example limonene) in the upper half of the chromatogram or mono and sesquiterpene alcohols (for example terpinen-4-ol) in the lower half of chromatograms. Similar results were also obtained for J. communis essential oils.
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