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  • 1 R.C. Patel Institute of Pharmaceutical Education and Research Karwand Naka, Shirpur Dist. Dhule 425 405 (M.S.) India
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Asimple, selective, precise, and stability-indicating HPTLC method for analysis of brimonidine tartrate as the bulk drug and in formulations has been established and validated. Chromatography on aluminum foil silica gel 60F254 plates with methanol-toluene-triethylamine 1:3.5:0.2 (ν/ν) as mobile phase gave a compact band at RF 0.48 ± 0.02. Densitometric analysis was performed in absorbance mode at 247 nm. Linear regression analysis of peak-area calibration data revealed a good linear relationship with r2 = 0.9965 ± 0.0013 in the concentration range 100–600 ng per band. The mean value ± SD of slope and intercept were 9.047 ± 0.11 and 553.0 ± 39.06. The method was validated for precision, recovery, and robustness. The limits of detection and quantitation were 9.40 and 28.51 ng per band, respectively. Brimonidine tartrate was subjected to hydrolysis (in acid, alkali, and neutral solutions), oxidation, and photodegradation, and was degraded under all these conditions. Statistical analysis proved the method enables selective, precise, and accurate identification and quantitative analysis of brimonidine tartrate as the bulk drug and in dosage forms.

  • The Merck Index: An encyclopedia of chemicals, drugs and biologicals, 14th edn., Merck and Co. Inc., Whitehouse Station, N.J., 1996, p. 225.

    '', in An encyclopedia of chemicals, drugs and biologicals , (1996 ) -.

  • S.C. Sweetman, Martindale: The Complete Drug Reference, 35th edn., Pharmaceutical Press, London, 2006, p. 1718.

    Sweetman S.C. , '', in Martindale: The Complete Drug Reference , (2006 ) -.

  • R.R. Shirke, N. Pai, Indian Drugs 39 (2002) 484–486.

    Pai N. , '' (2002 ) 39 Indian Drugs : 484 -486.

  • J. Ni, J. Rowe, T. Heidelbaugh, S. Sinha, A. Acheampong, Xenobiotica 37 (2007) 205–220.

    Sinha S. , '' (2007 ) 37 A. Acheampong, Xenobiotica : 205 -220.

  • S. Jiang, A.K. Chappa, J.W. Proksch, J. Chromatogr. B 877 (2009) 107–114.

    Proksch J.W. , '' (2009 ) 877 J. Chromatogr. B : 107 -114.

  • L. Kvapil, M. Grepl, P. Hradil, Acta Univ. Palacki. Olomuc. Fac. Rer. Nat. Chemica 42 (2003) 19–26.

    Hradil P. , '' (2003 ) 42 Acta Univ. Palacki. Olomuc. Fac. Rer. Nat. Chemica : 19 -26.

  • D.N. Tzovolou, F. Lamari, E.K. Mela, S.P. Gartaganis, N.K. Karamanos, Biomed. Chromatogr. 14 (2000) 301–305.

    Karamanos N.K. , '' (2000 ) 14 Biomed. Chromatogr. : 301 -305.

  • M.S. Ali, A.R. Khatri, M.I. Munir, M. Ghori, Chromatographia 70 (2009) 539–544.

    Ghori M. , '' (2009 ) 70 Chromatographia : 539 -544.

  • A. Acheampong, D.D. Tang-Liu, J. Pharm. Biomed. Anal. 3 (1995) 995–1002.

    Tang-Liu D.D. , '' (1995 ) 3 J. Pharm. Biomed. Anal. : 995 -1002.

  • M. Bakshi, S. Singh, J. Pharm. Biomed. Anal. 28 (2002) 1011–1040.

    Singh S. , '' (2002 ) 28 J. Pharm. Biomed. Anal. : 1011 -1040.

  • Q1 (A) R2, Stability testing of new drug substances and products, International Conference on Harmonization, IFPMA, Geneva, February 2003.

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