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Jessica Counihan Lafayette College Department of Chemistry Easton PA 18042 USA

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Kathryn Hueglin Lafayette College Department of Biology Easton PA 18042 USA

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Crystal Wagner Lafayette College Department of Biology Easton PA 18042 USA

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Stephen Gadomski Lafayette College Department of Biology Easton PA 18042 USA

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Peter Zani Lafayette College Department of Biology Easton PA 18042 USA

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Bernard Fried Lafayette College Department of Biology Easton PA 18042 USA

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Joseph Sherma Lafayette College Department of Chemistry Easton PA 18042 USA

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Diapause in insects typically results in metabolic adjustments that may include the levels of feeding and activity, metabolic rate, and the accumulation and utilization of reserves. The objective of this study was to characterize and quantify the various neutral lipid classes in the pitcher-plant mosquito (Wyeomyia smithii) that are associated with photoperiod-induced diapause. We used high-performance thin-layer chromatography (HPTLC) with 10 cm × 20 cm silica gel plates, petroleum ether-diethyl ether-glacial acetic acid 80:20:1 as the mobile phase, and 5% ethanolic phosphomolybdic acid solution as detection reagent to determine the percentage of replicate larvae samples (consisting of 16–31 larvae) that contained free sterols (FS), free fatty acids (FFA), triacylglycerols (TG), methyl esters (ME), and steryl esters (SE). Irrespective of the growth treatment (shortdays or long-days), the greatest concentrations of neutral lipids in all samples examined were TG. The effects of day length were such that during long-day (diapause terminating) conditions larvae contained less TG, more FS, and more FFA than larvae exposed to short-day (diapause inducing) conditions. Furthermore, the effects of body mass affected our results such that larger larvae contained more TG when in diapause, but less TG when not in diapause. Both ME and SE were only found in trace amounts in the short-day mosquitoes and not at all in long-day group. Our results are consistent with previous research that suggests TGs are important storage lipids during insect diapause.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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