Authors:
Károly Takács Fermentia Ltd Berlini út 47-49 1045 Budapest Hungary

Search for other papers by Károly Takács in
Current site
Google Scholar
PubMed
Close
,
Antal Szabó

Search for other papers by Antal Szabó in
Current site
Google Scholar
PubMed
Close
,
Irén Winkler BioFil Ltd Váci St. 87 1139 Budapest Hungary

Search for other papers by Irén Winkler in
Current site
Google Scholar
PubMed
Close
, and
Balázs Erdélyi

Search for other papers by Balázs Erdélyi in
Current site
Google Scholar
PubMed
Close
Restricted access

A simple thin-layer chromatography (TLC) method for continuous determination of metabolism of carbohydrate and production of polysaccharides in the course of bacterial fermentations is presented in this paper. Samples were taken from the supernatants of decanted fermentation broth and from the hydrolysates of both the supernatants and the fermentation broth. Hydrolysis of the exo-polysaccharides (EPSs) to the constituent monosaccharides was carried out by 10% v/v hydrochloric acid at 100°C. These samples were applied directly onto a precoated nano silica plate besides a series of mono-, oligo-, and polysaccharide reference solutions.The plates were developed in a twin-trough chamber previously saturated with a solution of chloroform-toluene-35% formic acid-methanol (10:2:2:7 v/v) for 1 h. The plates were placed in the empty trough of the chamber in order to be saturated and then transferred to the trough containing the solvent. After reaching a migration distance of 160 mm, the plates were dried out and placed in a chamber saturated with I2 vapor in order to detect starch-type polysaccharides.After drying, the plates were dipped into a dish containing a solution of 10% v/v concentrated sulfuric acid in n-propanol-toluene (1:1 v/v). The excess reagent was eliminated with filter papers. Visualization was achieved in an electric oven at 115°C for 10 min. Evaluation was accomplished by comparison of the RF values, and the intensities of the spots were evaluated instrumentally using a CAMAG TLC Scanner II. The presented method is suitable for monitoring the bacterial EPS production and determining the monosaccharide constituents.

  • P.M. Huang, J. Berthelin, J.-M. Bollag, W.B. McGill, A.L. Page, Environmental Impact of Soil Components Interactions, CRC Press, 1995.

  • A. Khanafari, A. Akhavan Sepahei, Int. J. Environ. Sci. Tech. 4 (2007) 1735–1742.

    Akhavan Sepahei A. , '' (2007 ) 4 Int. J. Environ. Sci. Tech. : 1735 -1742 .

  • Y.W. Han, M.A. Watson, J. Ind. Microbiol. 9 (1992) 257–260.

    Watson M.A. , '' (1992 ) 9 J. Ind. Microbiol. : 257 -260 .

  • I.Y. Lee, W.T. Seo, G.J. Kim, M.K. Kim, G.S. Kwon, Y.H. Park, Bioprocess Eng. 16 (1997) 71–75.

    Park Y.H. , '' (1997 ) 16 Bioprocess Eng. : 71 -75 .

  • N.K. Kilic, G. Dönmez, J. Hazard Mater. 154 (2008) 1019–1024.

    Dönmez G. , '' (2008 ) 154 J. Hazard Mater. : 1019 -1024 .

  • A. Szabó, A. Kónya, I. Winkler, Gy. Máté, B. Erdélyi, J. Planar Chromatogr. 19 (2006) 418–421.

    Erdélyi B. , '' (2006 ) 19 J. Planar Chromatogr. : 418 -421 .

  • K. Fukuda, T. Shi, K. Nagami, F. Leo, T. Nakamura, K. Yasuda, A. Senda, H. Motoshima, T. Urashima, Carbohydr. Polym. 79 (2010) 1040–1045.

    Urashima T. , '' (2010 ) 79 Carbohydr. Polym. : 1040 -1045 .

  • S. Meisen, J. Wingender, U. Telgheder, Anal. Bioanal. Chem. 391 (2008) 993–1002.

    Telgheder U. , '' (2008 ) 391 Anal. Bioanal. Chem. : 993 -1002 .

  • R. Verhoef, P. de Waard, H.A. Schols, M. Ratto, M. Siika-aho, A.G.J. Voragena, Carbohydr. Res. 337 (2002) 1821–1831.

    Voragena A.G.J. , '' (2002 ) 337 Carbohydr. Res. : 1821 -1831 .

  • J.K. Yan, L. Li, Z.M. Wanga, P.H. Leung, W.Q. Wangb, J.Y. Wub, Food Chem. 117 (2009) 641–646.

    Wub J.Y. , '' (2009 ) 117 Food Chem. : 641 -646 .

  • J.H. Yu, Y.M. Shin, K.S. Rhee, US Patent Nr. 5,420,036 (1995).

  • Y.W. Han, M.A. Clarke, US Patent Nr. 5,547,863 (1996).

  • Collapse
  • Expand

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Jan 2024 4 1 0
Feb 2024 18 0 0
Mar 2024 4 0 0
Apr 2024 10 0 0
May 2024 1 0 0
Jun 2024 2 0 0
Jul 2024 0 0 0