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Sigrid Mennickent University of Concepción Department of Pharmacy, Faculty of Pharmacy P.O. Box 237 Concepción Chile

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Ricardo Fierro University of Concepción Department of Pharmacy, Faculty of Pharmacy P.O. Box 237 Concepción Chile

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Mario Vega University of Concepción Department of Bromatology, Nutrition, and Dietetics, Faculty of Pharmacy P.O. Box 237 Concepción Chile

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Marta Diego University of Concepción Department of Pharmacy, Faculty of Pharmacy P.O. Box 237 Concepción Chile

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C. Godoy University of Concepción Department of Pharmacy, Faculty of Pharmacy P.O. Box 237 Concepción Chile

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An HPTLC method with densitometric detection at 280 nm has been established for quantification of lamotrigine in human serum. Liquid-liquid extraction with ethyl acetate, with chloramphenicol as internal standard, was followed by chromatography on silica gel F254 plates with ethyl acetate-methanol-32% aqueous ammonia 17:2:1 (v/v) as mobile phase. The method was validated for linearity, precision, and accuracy. Calibration plots were linear in the range 0.6 to 300 ng per band, corresponding to 0.06–30.00 ng μL−1 lamotrigine in human serum after extraction and application of 1 μL to the chromatographic plate. The correlation coefficient was 0.998. Intra-assay and inter-assay precision, as relative standard deviation (RSD), were in the range 0.53–2.91% (n = 3) and 1.58–2.98% (n = 9), respectively. The limits of detection and quantification were 0.016 and 0.042 ng, respectively. Accuracy, calculated as percentage recovery, was between 94.09 and 101.30%, with RSD no higher than 3.52%. The method was selective for both lamotrigine and the internal standard. The method enables rapid, precise, accurate, selective, and sensitive quantitative analysis of lamotrigine in human serum.

  • J. McNamara, in: J. Hardman, L. Limbird (eds.), Las Bases Farmacológicas de la Terapéutica, McGraw-Hill, México, 2003.

    McNamara J. , '', in Las Bases Farmacológicas de la Terapéutica , (2003 ) -.

  • G.K. McEvoy (ed.), AHFS Drug Information, American Society of Health-System Pharmacists, Bethesda, 2009.

    '', in AHFS Drug Information , (2009 ) -.

  • S. Sweetman, Martindale, Guía Completa de Consulta Farmacoterapéutica, Pharma Editores S.L, Barcelona, 2003.

    Sweetman S. , '', in Martindale, Guía Completa de Consulta Farmacoterapéutica , (2003 ) -.

  • J. Zheng, M. Jann, Y. Yi Hon, S. Shamsi, Electrophoresis 25 (2004) 2033–2043.

    Shamsi S. , '' (2004 ) 25 Electrophoresis : 2033 -2043 .

  • E. Greiner-Sosanko, D. Lower, M. Virji, M. Krasowski, Biomed. Chromatogr. 21 (2007) 225–228.

    Krasowski M. , '' (2007 ) 21 Biomed. Chromatogr. : 225 -228 .

  • C.L. Cheng, C.H. Chou, H. Oy, J. Chromatogr. B 87 (2005) 199–206.

    Oy H. , '' (2005 ) 87 J. Chromatogr. B : 199 -206 .

  • D. Croci, A. Salmaggi, U. de Grazia, G. Bernardi, Ther. Drug Monit. 23 (2001) 665–668.

    Bernardi G. , '' (2001 ) 23 Ther. Drug Monit. : 665 -668 .

  • R. Das Gupta, J. Guest, Br. J. Psychiatry 180 (2002) 227–233.

    Gupta R. , '' (2002 ) 180 J. Guest, Br. J. Psychiatry : 227 -233 .

  • A. Cooper, New study highlights impact of bipolar disorder on society. In: www.bipolarsupporter.com

  • L. Copeland, J. Zeber, I. Salloum, H. Pincus, M.J. Fine, A. Kilbourne, J. Nerv. Ment. Dis. 196 (2008) 16–21.

    Kilbourne A. , '' (2008 ) 196 J. Nerv. Ment. Dis. : 16 -21 .

  • F. Johnson, S. Ozdemir, R. Manjunath, B. Hauber, S. Burch, T. Thompson, Med. Care 45 (2007) 545–552.

    Thompson T. , '' (2007 ) 45 Med. Care : 545 -552 .

  • J. Clatworthy, R. Bowskill, T. Rank, R. Parham, R. Horne, Bipolar Disord. 9 (2007) 656–654.

    Horne R. , '' (2007 ) 9 Bipolar Disord. : 656 -654 .

  • R.S. Ell-Mallakh, J. Psychiatr. Pract. 13 (2007) 79–85.

    Ell-Mallakh R.S. , '' (2007 ) 13 J. Psychiatr. Pract. : 79 -85 .

  • M. Sajatovic, M. Valenstein, F. Blow, D. Ganoczy, R.V. Ignacio, Psychiatr. Serv.58 (2007) 855–863.

    Ignacio R.V. , '' (2007 ) 58 Psychiatr. Serv. : 855 -863 .

  • B. Gaudiano, Behav. Modif. 32 (2008) 267–301.

    Gaudiano B. , '' (2008 ) 32 Behav. Modif. : 267 -301 .

  • M. Sajatovic, M. Valenstein, F.C. Blow, D. Ganoczy, R.V. Ignacio, Bipolar Disord. 8 (2006) 232–241.

    Ignacio R.V. , '' (2006 ) 8 Bipolar Disord. : 232 -241 .

  • M. Hassan, Ann. Pharmacother. 41 (2007) 1812–1818.

    Hassan M. , '' (2007 ) 41 Ann. Pharmacother. : 1812 -1818 .

  • C. Depp, B. Lebowitz, T. Patterson, J. Lacro, V.J. Dillip, Bipolar Disord. 9 (2007) 636–645.

    Dillip V.J. , '' (2007 ) 9 Bipolar Disord. : 636 -645 .

  • L. Osterbergs, T. Blachske, New Engl. J. Med. 353 (2005) 487–497.

    Blachske T. , '' (2005 ) 353 New Engl. J. Med. : 487 -497 .

  • M. Sajatovic, P.J. Chen, P. Dines, E. Shirley, Dis. Manag. Health Out.15 (2007) 181–192.

    Shirley E. , '' (2007 ) 15 Dis. Manag. Health Out. : 181 -192 .

  • E. Greiner-Sosanko, D. Lower, M. Virji, M. Krasowski, Biomed. Chromatogr.21 (2007) 225–228.

    Krasowski M. , '' (2007 ) 21 Biomed. Chromatogr. : 225 -228 .

  • C.L. Cheng, C.H. Chou, J. Chromatogr. B817 (2005) 199–206.

    Chou C.H. , '' (2005 ) 817 J. Chromatogr. B : 199 -206 .

  • T.A. Vermeij, P.M. Edelbroek, J. Chromatogr. B 357 (2007) 40–46.

    Edelbroek P.M. , '' (2007 ) 357 J. Chromatogr. B : 40 -46 .

  • L. Francsechi, M. Furlanut, Pharmacol. Res. 51 (2005) 297–302.

    Furlanut M. , '' (2005 ) 51 Pharmacol. Res. : 297 -302 .

  • G. Marcelín-Jiménez, A. Citlali, A. Moreno, L. Mendoza-Morales, L. Rivera-Espinosa, M. Morales Martínez, Bioanalysis 1 (2009) 47–55.

    Morales Martínez M. , '' (2009 ) 1 Bioanalysis : 47 -55 .

  • P. Kuldeep, B. Subhash, J. Chromatogr. B 823 (2005) 152–157.

    Subhash B. , '' (2005 ) 823 J. Chromatogr. B : 152 -157 .

  • I. Wilson, Ther. Drug Monit. 18 (1996) 484–492.

    Wilson I. , '' (1996 ) 18 Ther. Drug Monit. : 484 -492 .

  • S. Mennickent, A. Sobarzo, M. Vega, C.G. Godoy, M. de Diego, J. Sep. Sci. 30 (2007) 2167–2172.

    Diego M. , '' (2007 ) 30 J. Sep. Sci. : 2167 -2172 .

  • S. Mennickent, R. Fierro, M. Vega, M. de Diego, J. Sep. Sci. 32 (2009) 1454–1458.

    Diego M. , '' (2009 ) 32 J. Sep. Sci. : 1454 -1458 .

  • S. Mennickent, A. Sobarzo, M. Vega, M. de Diego, G. Godoy, P. Rioseco, L. Saavedra, Int. J. Psychiatr. Clin. Pract. 14 (2010) 41–46.

    Saavedra L. , '' (2010 ) 14 Int. J. Psychiatr. Clin. Pract. : 41 -46 .

  • S. Mennickent, M. Vega, C.G. Godoy, M.D. León, Rev. Méd. Chile 135 (2007) 335–340.

    León M.D. , '' (2007 ) 135 Rev. Méd. Chile : 335 -340 .

  • S. Mennickent, L. Pino, M. Vega, C.G. Godoy, M. de Diego, J. Sep. Sci. 30 (2007) 772–777.

    Diego M. , '' (2007 ) 30 J. Sep. Sci. : 772 -777 .

  • S. Mennickent, L. Pino, M. Vega, M. de Diego, J. Sep. Sci. 31 (2007) 201–206.

    Diego M. , '' (2007 ) 31 J. Sep. Sci. : 201 -206 .

  • S. Mennickent, M. Nail, M. Vega, M. de Diego, J. Sep. Sci. 30 (2007) 1893–1898.

    Diego M. , '' (2007 ) 30 J. Sep. Sci. : 1893 -1898 .

  • S. Mennickent, J. Contreras, C. Reyes, M. Vega, M. de Diego, J. Planar Chromatogr. 23 (2010) 1–13.

    Diego M. , '' (2010 ) 23 J. Planar Chromatogr. : 1 -13 .

  • G. Shabir, J. Chromatogr. A987 (2003) 57–66

    Shabir G. , '' (2003 ) 987 J. Chromatogr. A : 57 -66 .

  • The United States Pharmacopeia, 29th edn., U.S. Pharmacopeial Convention Inc., Rockville, 2005.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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