An HPTLC method with densitometric detection at 280 nm has been established for quantification of lamotrigine in human serum. Liquid-liquid extraction with ethyl acetate, with chloramphenicol as internal standard, was followed by chromatography on silica gel F254 plates with ethyl acetate-methanol-32% aqueous ammonia 17:2:1 (v/v) as mobile phase. The method was validated for linearity, precision, and accuracy. Calibration plots were linear in the range 0.6 to 300 ng per band, corresponding to 0.06–30.00 ng μL−1 lamotrigine in human serum after extraction and application of 1 μL to the chromatographic plate. The correlation coefficient was 0.998. Intra-assay and inter-assay precision, as relative standard deviation (RSD), were in the range 0.53–2.91% (n = 3) and 1.58–2.98% (n = 9), respectively. The limits of detection and quantification were 0.016 and 0.042 ng, respectively. Accuracy, calculated as percentage recovery, was between 94.09 and 101.30%, with RSD no higher than 3.52%. The method was selective for both lamotrigine and the internal standard. The method enables rapid, precise, accurate, selective, and sensitive quantitative analysis of lamotrigine in human serum.
J. McNamara, in: J. Hardman, L. Limbird (eds.), Las Bases Farmacológicas de la Terapéutica, McGraw-Hill, México, 2003.
McNamara J., '', in Las Bases Farmacológicas de la Terapéutica, (2003) -.
McNamara J.Las Bases Farmacológicas de la Terapéutica2003)| false
G.K. McEvoy (ed.), AHFS Drug Information, American Society of Health-System Pharmacists, Bethesda, 2009.
'', in AHFS Drug Information, (2009) -.
AHFS Drug Information2009)| false
S. Sweetman, Martindale, Guía Completa de Consulta Farmacoterapéutica, Pharma Editores S.L, Barcelona, 2003.
Sweetman S., '', in Martindale, Guía Completa de Consulta Farmacoterapéutica, (2003) -.
Sweetman S.Martindale, Guía Completa de Consulta Farmacoterapéutica2003)| false
J. Zheng, M. Jann, Y. Yi Hon, S. Shamsi, Electrophoresis 25 (2004) 2033–2043.