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Nitin Shelke R.C. Patel Institute of Pharmaceutical Education and Research Bioecology Laboratory, Department of Pharmacognosy Shirpur 425405 India

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Vinodkumar Kuber Analytical R&D, Tulip Lab Pvt. Ltd. Plot No-F-20/21, MIDC Ranjangaon Tal-Shirur, Dist-Pune 412220 India

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Shrinivas Bhope Analytical R&D, Tulip Lab Pvt. Ltd. Plot No-F-20/21, MIDC Ranjangaon Tal-Shirur, Dist-Pune 412220 India

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Ramchandra Jadhav R.C. Patel Institute of Pharmaceutical Education and Research Bioecology Laboratory, Department of Pharmacognosy Shirpur 425405 India

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A simple, precise, and rapid HPTLC method has been established for simultaneous analysis of E-guggulsterone, Z-guggulsterone, 11-keto-β-boswellic acid (11-KBA), and 3-acetyl-11-keto-β-boswellic acid (A-11-KBA) in an anti-arthritic formulation. The method involves densitometric evaluation of E and Z-guggulsterone, 11-KBA and A-11-KBA after their resolution on pre-coated silica gel 60F254 plates with n-hexane-chloroform-ethyl acetate-methanol 10:3:3:1 (ν/ν) as mobile phase. Detection was at 254 nm. Good resolution was achieved, with RF 0.61 ± 0.03, 0.68 ± 0.03, 0.28 ± 0.03, and 0.39 ± 0.03 for E and Z-guggulsterone 11-KBA, and A-11-KBA, respectively. The method was validated for specificity, linearity, precision, accuracy, and robustness. The method was linear in the range 10–90 ng per band for E and Z-guggulsterone and 50–450 ng per band for 11-KBA and A-11-KBA. Recovery at 80, 100, and 120% was 96.93 ± 0.27, 97.39 ± 0.22, 97.60 ± 0.61, and 97.19 ± 0.41% for E- and Z-guggulsterone, 11-KBA, and A-11-KBA, respectively. The method is simple, specific, precise, accurate, reproducible, and gives results comparable with those from an HPLC method.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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