Authors:
Marek Ellnain Jagiellonian University Department of Pharmacognosy, Medical College 9, Medyczna Street Cracow 30-688 Poland

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Urszula Hubicka Jagiellonian University Department of Inorganic and Analytical Chemistry, Medical College 9, Medyczna Street Cracow 30-688 Poland

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Barbara Żuromska Jagiellonian University Department of Inorganic and Analytical Chemistry, Medical College 9, Medyczna Street Cracow 30-688 Poland

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Zbigniew Janeczko Jagiellonian University Department of Pharmacognosy, Medical College 9, Medyczna Street Cracow 30-688 Poland

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Jan Krzek Jagiellonian University Department of Inorganic and Analytical Chemistry, Medical College 9, Medyczna Street Cracow 30-688 Poland

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Erigeron canadensis is an annual plant of the Asteraceae family, widely distributed in rural and urban areas of Poland and other European countries, which has been reported to be resistant to almost all kinds of herbicide. It has been used in folk medicine to treat diarrhea and as a hemostatic agent, and also has anti-inflammatory, diuretic, and antiseptic activity. The plant is reported to contain tannins, flavonoids, phenolic acids, and sphingolipids; its essential oil is rich in limonene and β-ocimene. A simple densitometric TLC method has been established for analysis of monogalactosyldiacylglycerol (MGDG) and digalactosyldiacylglycerol (DGDG) in extracts of fresh samples of Erigeron canadensis collected at different stages of growth. Silica gel 60 was used as stationary phase and chloroform-methanol-10% acetic acid 16:4:0.4 (ν/ν) as mobile phase. Detection and quantification were performed densitometrically at 477 nm, after derivatization by spraying with a 25% solution of sulfuric acid in methanol and heating at 105°C for 5 min. The method was validated for specificity, linearity, precision, recovery, and limits of detection and quantification.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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