Authors:
Amar Singh JN Custom House Customs Laboratory Nhava Sheva, Navi-Mumbai 400 707 India

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Naved Alvi Gandhi Faizam Degree College Shahjahanpur 240 001 UP India

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A simple, sensitive, and reliable normal-phase HPTLC method has been established for quantification of yohimbine in the stem bark of P. yohimbe. A methanol extract of powdered stem bark was used for quantification. Chromatography was performed on silica gel F254 HPTLC plates with toluene-ethyl acetate-diethyl amine 7:2:1 (ν/ν) as mobile phase. Quantification was achieved by densitometric scanning at 285 nm in reflectance-absorbance mode. The response to yohimbine was a linear function of concentration over the range 400 to 1200 ng per band. The mean amount of yohimbine in samples of the stem bark of P. yohimbe was 0.56 mg g−1. The method was validated for selectivity, linearity, accuracy, recovery, precision, and robustness.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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