JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 24: Issue 3
Simultaneous analysis of hydrophilic and lipophilic compounds in Salvia miltiorrhiza by double-development HPTLC and scanning densitometry
Authors:
Jing Yang University of Macau State Key Laboratory for Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences Macao SAR China

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Lei-lei Choi University of Macau State Key Laboratory for Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences Macao SAR China

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De-qiang Li University of Macau State Key Laboratory for Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences Macao SAR China

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Feng-qing Yang University of Macau State Key Laboratory for Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences Macao SAR China

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Ling-jie Zeng Guangdong Pharmaceutical University Faculty of Chinese Medicine Guangzhou China

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Jing Zhao University of Macau State Key Laboratory for Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences Macao SAR China

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Shao-ping Li University of Macau State Key Laboratory for Quality Research in Chinese Medicine and Institute of Chinese Medical Sciences Macao SAR China

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Pages:
257–263
Online Publication Date:
02 Jun 2011
Publication Date:
01 Jun 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.24.2011.3.16
Keywords:
HPTLC-densitometry; Salvia miltiorrhiza ; Phenolic acids; Tanshinones; Pressurized liquid extraction (PLE)
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A double-development TLC method has been developed for simultaneous qualitative and quantitative analysis of hydrophilic and lipophilic constituents of Salvia miltiorrhiza (Danshen). The optimized mobile phases dichloromethane-ethyl acetate-formic acid 22:24:10 (ν/ν) and petroleum ether-ethyl acetate-cyclohexane 25:11:14 (ν/ν) were used for the double development on nano-silica gel 60F254 plates. Their characteristic TLC profiles were observed under UV light at 254 and 365 nm and the bands were then revealed by reaction with 5% H2SO4 in EtOH. Quantification of twelve compounds was achieved by densitometry at 260 or 290 nm, with reference at 400 nm. Linearity was quite good (R2 > 0.99) within the ranges tested. This method could be used for quality control of Danshen.

  • X. Wang, S.L. Morris-Natschke, K.H. Lee, Med. Res. Rev. 27 (2007) 133–148.

    Lee K.H. , '' (2007 ) 27 Med. Res. Rev. : 133 -148 .

  • R.W. Jiang, K.M. Lau, P.M. Hon, T.C.W. Mak, K.S. Woo, K.P. Fung, Curr. Med. Chem. 12 (2005) 237–246.

    Fung K.P. , '' (2005 ) 12 Curr. Med. Chem. : 237 -246 .

  • L.M. Zhou, Z. Zuo, M.S.S. Chow, J. Clin. Pharmacol. 45 (2005) 1345–1359.

    Chow M.S.S. , '' (2005 ) 45 J. Clin. Pharmacol. : 1345 -1359 .

  • Y.G. Li, L. Song, M. Liu, Z.B. Hu, Z.T. Wang, J. Chromatogr. A 1216 (2009) 1941–1953.

    Wang Z.T. , '' (2009 ) 1216 J. Chromatogr. A : 1941 -1953 .

  • M. Gu, Z.G. Su, F. Ouyang, J. Liq. Chromatogr. Relat. Technol. 29 (2006) 1503–1514.

    Ouyang F. , '' (2006 ) 29 J. Liq. Chromatogr. Relat. Technol. : 1503 -1514 .

  • P. Hu, G.A. Luo, Z.Z. Zhao, Z.H. Jiang, Chem. Pharm. Bull. 53 (2005) 481–486.

    Jiang Z.H. , '' (2005 ) 53 Chem. Pharm. Bull. : 481 -486 .

  • X.J. Pan, G.G. Niu, H.Z. Liu, J. Chromatogr. A 922 (2001) 371–375.

    Liu H.Z. , '' (2001 ) 922 J. Chromatogr. A : 371 -375 .

  • H.B. Qu, X.J. Zhai, Q. Shao, Y.Y. Cheng, Chromatographia 66 (2007) 21–27.

    Cheng Y.Y. , '' (2007 ) 66 Chromatographia : 21 -27 .

  • D. Yuan, Y.N. Pan, W.W. Fu, T. Makino, Y. Kano, Chem. Pharm. Bull. 53 (2005) 508–514.

    Kano Y. , '' (2005 ) 53 Chem. Pharm. Bull. : 508 -514 .

  • A.H. Liu, L. Li, M. Xu, Y.H. Lin, H.Z. Guo, D.A. Guo, J. Pharm. Biomed. Anal. 41 (2006) 48–56.

    Guo D.A. , '' (2006 ) 41 J. Pharm. Biomed. Anal. : 48 -56 .

  • M. Gu, S.F. Zhang, Z.G. Su, Y. Chen, F. Ouyang, J. Chromatogr. A 1057 (2004) 133–140.

    Ouyang F. , '' (2004 ) 1057 J. Chromatogr. A : 133 -140 .

  • P. Li, G. Xu, S.P. Li, Y.T. Wang, T.P. Fan, Q.S. Zhao, Q.W. Zhang, J. Agric. Food Chem. 56 (2008) 1164–1171.

    Zhang Q.W. , '' (2008 ) 56 J. Agric. Food Chem. : 1164 -1171 .

  • M. Liu, Y.G. Li, G.X. Chou, X.M. Cheng, M. Zhang, Z.T. Wang, J. Chromatogr. A 1157 (2007) 51–55.

    Wang Z.T. , '' (2007 ) 1157 J. Chromatogr. A : 51 -55 .

  • X.M. Liu, J.S. Zhang, X.G. Chen, J. Chromatogr. B 852 (2007) 325–332.

    Chen X.G. , '' (2007 ) 852 J. Chromatogr. B : 325 -332 .

  • H.B. Li, F. Chen, J. Chromatogr. A 925 (2001) 109–114.

    Chen F. , '' (2001 ) 925 J. Chromatogr. A : 109 -114 .

  • H.B. Li, J.P. Lai, Y. Jiang, F. Chen, J. Chromatogr. A 943 (2002) 235–239.

    Chen F. , '' (2002 ) 943 J. Chromatogr. A : 235 -239 .

  • M. Gu, G.F. Zhang, Z.G. Su, F. Ouyang, J. Chromatogr. A 1041 (2004) 239–243.

    Ouyang F. , '' (2004 ) 1041 J. Chromatogr. A : 239 -243 .

  • R. Vijayakannan, M. Karan, S. Dutt, V. Jain, K. Vasisht, Chromatographia 63 (2006) 277–281.

    Vasisht K. , '' (2006 ) 63 Chromatographia : 277 -281 .

  • E. Bodoki, R. Oprean, L. Vlase, M. Tamas, R. Sandulescu, J. Pharm. Biomed. Anal. 37 (2005) 971–977.

    Sandulescu R. , '' (2005 ) 37 J. Pharm. Biomed. Anal. : 971 -977 .

  • I. Fecka, S. Turek, Food. Chem. 108 (2008) 1039–1053.

    Turek S. , '' (2008 ) 108 Food. Chem. : 1039 -1053 .
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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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