JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 24: Issue 4
Quantitative analysis of flavonoids in Annona squamosa leaf extracts and its pellet formulation by validated high-performance thin-layer chromatographic technique
Authors:
Neha Desai Bombay College of Pharmacy Department of Pharmaceutics Kalina, Santacruz (E), Mumbai 400098 India

Search for other papers by Neha Desai in
Current site
Google Scholar
PubMed Close
,
Chandrashekhar Barhate Bombay College of Pharmacy Department of Pharmaceutical Chemistry Kalina, Santacruz (E), Mumbai 400098 India

Search for other papers by Chandrashekhar Barhate in
Current site
Google Scholar
PubMed Close
,
Sheetal Biyani Bombay College of Pharmacy Department of Pharmacognosy and Phytochemistry Kalina, Santacruz (E), Mumbai 400098 India

Search for other papers by Sheetal Biyani in
Current site
Google Scholar
PubMed Close
,
Savita Kulkarni Bombay College of Pharmacy Department of Pharmacognosy and Phytochemistry Kalina, Santacruz (E), Mumbai 400098 India

Search for other papers by Savita Kulkarni in
Current site
Google Scholar
PubMed Close
, and
Mangal Nagarsenker Bombay College of Pharmacy Department of Pharmaceutics Kalina, Santacruz (E), Mumbai 400098 India

Search for other papers by Mangal Nagarsenker in
Current site
Google Scholar
PubMed Close
Pages:
306–311
Online Publication Date:
13 Aug 2011
Publication Date:
01 Aug 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.24.2011.4.6
Keywords:
Rutin; Isoquercitrin; Annona squamosa ; High-performance thin-layer chromatography; Herbal extracts; Pellets
Restricted access

Annona squamosa, commonly known as custard apple, possesses various medicinal properties such as antimicrobial, insecticidal, cardiotonic, etc. Leaves of A. squamosa are known to contain various types of flavonoids which are responsible for these activities. The aim of this article was to develop and validate a high-performance thin-layer chromatography (HPTLC) method for the determination of rutin and isoquercitrin in the extracts of A. squamosa and in an in-house developed herbal pharmaceutical formulation. Analysis was performed on TLC aluminum plates precoated with silica gel 60 F254 as the stationary phase. Linear ascending development was carried out in a twin-trough glass chamber saturated with a mobile phase consisting of ethyl acetate-formic acid-glacial acetic acid-ethyl methyl ketone-water 50:7:3:30:10 (ν/ν/ν/ν/ν) at room temperature (25 ± 2°C). A CAMAG TLC scanner III was used for the spectrodensitometric scanning at 366 nm. The system was found to give compact bands for rutin and isoquercitrin (RF values of 0.32 ± 0.05 and 0.59 ± 0.03, respectively). The detector response was linear for concentrations ranging between 200 and 1600 ng per band (r2 = 0.9885 ± 0.0041 for rutin and 0.9828 ± 0.0013 for isoquercitrin). The limits of detection and quantitation were 75 and 100 ng per band for rutin, and 40 and 80 ng per band for isoquercitrin, respectively. The recovery study was carried out by the standard addition method and was found to be 96–107%. The present method was found to be simple, specific, sensitive, and accurate and was used successfully to quantitate rutin and isoquercitrin in the leaf extracts of A. squamosa collected from five different locations of India and in a pellet formulation of the extract.

  • L.V. Asolkar, K.K. Kakkar, O.J. Chakre, Glossary of Indian Medicinal Plants with Active Principles, Publication and Information Directorate, New Delhi, 1992.

    Chakre O.J. , '', in Glossary of Indian Medicinal Plants with Active Principles , (1992 ) -.

  • D. Bhakuni, M. Dhar, M. Dhar, B. Dhawan, B. Mehrotra, Indian J. Exp. Biol. 7 (1969) 250–262.

    Mehrotra B. , '' (1969 ) 7 Indian J. Exp. Biol. : 250 -262 .

  • H. Kotkar, P. Mendki, S. Sadan, S. Jha, S. Upasani, V. Maheshwari, Pest. Manag. Sci. 58 (2002) 33–37.

    Maheshwari V. , '' (2002 ) 58 Pest. Manag. Sci. : 33 -37 .

  • T. Seetharaman, Fitoterapia 57 (1986) 189–198.

    Seetharaman T. , '' (1986 ) 57 Fitoterapia : 189 -198 .

  • R. Gupta, A. Kesari, P. Murthy, R. Chandra, V. Tandon, G. Watal, J. Ethnopharmacol. 99 (2005) 75–81.

    Watal G. , '' (2005 ) 99 J. Ethnopharmacol. : 75 -81 .

  • S. Panda, A. Kar, Biofactors 31 (2007) 201–210.

    Kar A. , '' (2007 ) 31 Biofactors : 201 -210 .

  • S. Castaldo, F. Capasso, Fitoterapia 73 (2002) 1–6.

    Capasso F. , '' (2002 ) 73 Fitoterapia : 1 -6 .

  • L. Liviero, P. Puglisi, P. Morazzoni, E. Bombardelli, Fitoterapia 65 (1993) 203–209.

    Bombardelli E. , '' (1993 ) 65 Fitoterapia : 203 -209 .

  • P. Bhandari, N. Kumar, A. Gupta, B. Singh, V. Kaul, J. Sep. Sci. 30 (2007) 2092–2096.

    Kaul V. , '' (2007 ) 30 J. Sep. Sci. : 2092 -2096 .

  • P. Poukens-Renwart, M. Tits, J.N. Wauters, L. Angenot, J. Pharm. Biomed. Anal. 10 (1992) 1085–1088.

    Angenot L. , '' (1992 ) 10 J. Pharm. Biomed. Anal. : 1085 -1088 .

  • J. Corthout, T. Naessens, S. Apers, A. J. Vlietinck, J. Pharm. Biomed. Anal. 21 (1999) 187–192.

    Vlietinck A. J. , '' (1999 ) 21 J. Pharm. Biomed. Anal. : 187 -192 .

  • M. Ravishankara, N. Srivastava, M. Jayathirtha, H. Padh, M. Rajani, J. Chromatogr. B 744 (2000) 257–262.

    Rajani M. , '' (2000 ) 744 J. Chromatogr. B : 257 -262 .

  • A. Swaroop, A.P. Gupta, A.K. Sinha, Chromatographia 62 (2005) 649–652.

    Sinha A.K. , '' (2005 ) 62 Chromatographia : 649 -652 .

  • H. Wagner, S. Bladt, Plant Drug Analysis. A Thin Layer Chromatography. Atlas Springer-Verlag, Berlin, 2002.

    Bladt S. , '', in Plant Drug Analysis. A Thin Layer Chromatography , (2002 ) -.

  • M. Tomczyk, A. Bazylko, A. Staszewska, Phytochem. Anal. 21 (2010) 174–179.

    Staszewska A. , '' (2010 ) 21 Phytochem. Anal. : 174 -179 .

  • S. Ahmad, M. Rizwan, R. Parveen, M. Mujeeb, M. Aquil, Chromatographia 67 (2008) 441–447.

    Aquil M. , '' (2008 ) 67 Chromatographia : 441 -447 .

  • H. Agarawal, N. Kaul, A. Paradkar, K. Mahadik, J. Pharm. Biomed. Anal. 36 (2004) 33–41.

    Mahadik K. , '' (2004 ) 36 J. Pharm. Biomed. Anal. : 33 -41 .

  • N. Sharma, U.K. Sharma, A.P. Gupta, A.K. Sinha, J. Food Comp. Anal. 23 (2010) 214–219.

    Sinha A.K. , '' (2010 ) 23 J. Food Comp. Anal. : 214 -219 .

  • M. Ansari, S. Ahmad, K. Kohli, J. Ali, R. Khar, J. Pharm. Biomed. Anal. 39 (2005) 132–138.

    Khar R. , '' (2005 ) 39 J. Pharm. Biomed. Anal. : 132 -138 .

  • ICH, Q2A (R1), Validation of Analytical Procedures: Text and Methodology, in: Proceedings of International Conference on Harmonization, Geneva, November 2005; http://www.ich.org/LOB/media/MEDIA417.pdf

  • Collapse
  • Expand
Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads