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Alok Lehri Council of Scientific and Industrial Research Central Instrumentation Facility, National Botanical Research Institute Lucknow 226 001 India

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Jyoti Barthwal Council of Scientific and Industrial Research Central Instrumentation Facility, National Botanical Research Institute Lucknow 226 001 India

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Abhishek Niranjan Council of Scientific and Industrial Research Central Instrumentation Facility, National Botanical Research Institute Lucknow 226 001 India

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Devendra Amla Council of Scientific and Industrial Research Central Instrumentation Facility, National Botanical Research Institute Lucknow 226 001 India

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India is the main producer of palmarosa oil obtained from rosha grass (Cymbopogan martini var. motia) of family Graminae. The essential oil obtained from the grass is rich in geraniol content. The oil is commercially obtained by hydro-steam distillation of rosha grass. Oil of palmarosa and its separated fraction geraniol are widely used in perfumery industry. The palmarosa oil is valued due to geraniol and largely used as base for fine perfumery. In this article, a simple, rapid, cost-effective and accurate method using high-performance thin-layer chromatographic (HPTLC) method has been developed for separation, identification and quantification of geraniol in palmarosa oil. Separation and quantification of palmarosa are achieved by HPTLC using mobile phase of toluene-ethylacetate (92.5:7.5) followed by separation on precoated silica gel 60 F254 aluminium plates and densitometric determination carried out after derivatization with vanillin-sulfuric acid reagent at λmax, 400 nm, in absorption-reflectance mode. The calibration curves were linear in the range of (1–7 μg) and all the distinguished bands were observed at 400 nm. The method was also evaluated for different validation parameters such as linearity, accuracy, precision, limit of detection (LOD), limit of quantification (LOQ), specificity, selectivity and sample stability. The amount of geraniol varied from 78.3% to 78.9% by HPTLC and 78.2% to 78.7% by gas chromatographyflame-ionization detector (GC-FID).

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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