Authors:
Dipak Kale RTM Nagpur University Department of Pharmaceutical Sciences Amravati Road Nagpur 440033 Maharashtra India

Search for other papers by Dipak Kale in
Current site
Google Scholar
PubMed
Close
and
Rajendra Kakde RTM Nagpur University Department of Pharmaceutical Sciences Amravati Road Nagpur 440033 Maharashtra India

Search for other papers by Rajendra Kakde in
Current site
Google Scholar
PubMed
Close
Restricted access

A simple, precise, and accurate high-performance thin-layer chromatography (HPTLC) method for the simultaneous determination of pioglitazone, metformin, and glimepiride in multicomponent pharmaceutical preparations has been developed and validated. Pioglitazone, metformin, and glimepiride from the formulations were separated on silica gel 60 F254 HPTLC plates with acetonitrile, methanol, propyl alcohol, and ammonium acetate solutions in the proportion of 7:2:1:1 (ν/ν) as mobile phase. Densitometric quantification was performed at 240 nm. Well-resolved bands were obtained with RF values 0.83, 0.21, and 0.89 for pioglitazone, metformin, and glimepiride, respectively. The method was validated for precision, accuracy, specificity, and robustness. The calibration curve was found to be linear in the concentration range of 0.3–1.2, 10–40, and 0.04–0.16 μg per band by area with correlation coefficients of 0.995, 0.996, and 0.998 for pioglitazone, metformin, and glimepiride, respectively. The method is selective and specific, with potential application in pharmaceutical analysis of these drugs in bulk and formulations.

  • P.B. Musholt, T. Schondorf, A. Pfutzner, C. Hohberg, I. Kleine, W. Fuchs, S. Hehenwarter, G. Dikta, B. Kerschgens, T. Forst, J. Diabetes. Sci. Technol. 3 (2009) 1442–1450.

    Forst T. , '' (2009 ) 3 J. Diabetes. Sci. Technol. : 1442 -1450 .

  • K. Aljabri, S.E. Kozak, D.M. Thompson, Am. J. Med. 116 (2004) 230–235.

    Thompson D.M. , '' (2004 ) 116 Am. J. Med. : 230 -235 .

  • E. Draeger, A.G. Hoechst, Diabetes Res. Clin. Pract. 28 (1995) 139–146.

    Hoechst A.G. , '' (1995 ) 28 Diabetes Res. Clin. Pract. : 139 -146 .

  • H. Ikeda, S. Taketomi, Y. Sugiyama, Y. Shimura, T. Sohda, K. Meguro, T. Fujita, Arzneimittelforschung 40 (1990) 156–162.

    Fujita T. , '' (1990 ) 40 Arzneimittelforschung : 156 -162 .

  • W.Z. Zhong, D.B. Lakings, J. Chromatogr. 490 (1989) 377–385.

    Lakings D.B. , '' (1989 ) 490 J. Chromatogr. : 377 -385 .

  • P. Sripalakit, P. Neamhom, A. Saraphanchotiwitthaya, Biomed. Chromatogr. 20 (2006) 1043–1048.

    Saraphanchotiwitthaya A. , '' (2006 ) 20 Biomed. Chromatogr. : 1043 -1048 .

  • E. Tahmasebi, Y. Yamini, A. Saleh, J. Chromatogr. B 877 (2009) 1923–1929.

    Saleh A. , '' (2009 ) 877 J. Chromatogr. B : 1923 -1929 .

  • E. Souri, H. Jalalizadeh, S. Saremi, J. Chromatogr. Sci. 46 (2008) 809–812.

    Saremi S. , '' (2008 ) 46 J. Chromatogr. Sci. : 809 -812 .

  • P. Sripalakit, P. Neamhom, A. Saraphanchotiwitthaya, J. Chromatogr. B 843 (2006) 164–169.

    Saraphanchotiwitthaya A. , '' (2006 ) 843 J. Chromatogr. B : 164 -169 .

  • A. Jedlicka, J. Klimes, A.T. Grafnetterov, Pharmazie 59 (2004) 178–182.

    Grafnetterov A.T. , '' (2004 ) 59 Pharmazie : 178 -182 .

  • T. Radhakrishna, D. Sreenivas Rao, G. Om Reddy, J. Pharm. Biomed. Anal. 29 (2002) 593–607.

    Reddy G. O. , '' (2002 ) 29 J. Pharm. Biomed. Anal. : 593 -607 .

  • Z.J. Lin, W. Ji, D. Desai-Krieger, L. Shum, J. Pharm. Biomed. Anal. 33 (2003) 101–108.

    Shum L. , '' (2003 ) 33 J. Pharm. Biomed. Anal. : 101 -108 .

  • P. Venkatesh, T. Harisudhan, H. Choudhury, R. Mullangi, N.R. Srinivas, Biomed. Chromatogr. 20 (2006) 1043–1048.

    Srinivas N.R. , '' (2006 ) 20 Biomed. Chromatogr. : 1043 -1048 .

  • P. Venkatesh, T. Harisudhan, H. Choudhury, R. Mullangi, N.R. Srinivas, J. Chromatogr. B 843 (2006) 164–169.

    Srinivas N.R. , '' (2006 ) 843 J. Chromatogr. B : 164 -169 .

  • K. Yamashita, H. Murakami, T. Okuda, M. Motohashi, J. Chromatogr. B 677 (1996) 141–146.

    Motohashi M. , '' (1996 ) 677 J. Chromatogr. B : 141 -146 .

  • M. Tubino, L.F. Bianchessi, M.M. Vila, Anal Sci. 26 (2010) 121–124.

    Vila M.M. , '' (2010 ) 26 Anal Sci. : 121 -124 .

  • J. Keal, A. Somogyi, J. Chromatogr. 378 (1986) 503–508.

    Somogyi A. , '' (1986 ) 378 J. Chromatogr. : 503 -508 .

  • A. Zarghi, S.M. Foroutan, A. Shafaati, A. Khoddam, J. Pharm. Biomed. Anal. 31 (2003) 197–200.

    Khoddam A. , '' (2003 ) 31 J. Pharm. Biomed. Anal. : 197 -200 .

  • S. Abu Ruz, J. Millership, J. Mc Elnay, J. Chromatogr. B 798 (2003) 203–209.

    Mc Elnay J. , '' (2003 ) 798 J. Chromatogr. B : 203 -209 .

  • J.Z. Song, H.F. Chen, S.J. Tian, Z.P. Sun, J. Chromatogr. B 708 (1998) 277–283.

    Sun Z.P. , '' (1998 ) 708 J. Chromatogr. B : 277 -283 .

  • I. Ali, H.Y. Aboul-Enein, V.K. Gupta, Comb. Chem. High Throughput Screen 10 (2007) 611–615.

    Gupta V.K. , '' (2007 ) 10 Comb. Chem. High Throughput Screen : 611 -615 .

  • R. Huupponen, P. Ojala-Karlsson, J. Rouru, M. Koulu, J. Chromatogr. 583 (1992) 270–273.

    Koulu M. , '' (1992 ) 583 J. Chromatogr. : 270 -273 .

  • B.G. Charles, N.W. Jacobsen, P.J. Ravenscroft, Clin. Chem. 27 (1981) 434–436.

    Ravenscroft P.J. , '' (1981 ) 27 Clin. Chem. : 434 -436 .

  • V. Porta, S.G. Schramm, E.K. Kano, E.E. Koono, Y.P. Armando, K. Fukuda, C.H. Serra, J. Pharm. Biomed. Anal. 46 (2008) 143–147.

    Serra C.H. , '' (2008 ) 46 J. Pharm. Biomed. Anal. : 143 -147 .

  • M.S. Arayne, N. Sultana, M.H. Zuberi, Pak. J. Pharm. Sci. 19 (2006) 231–235.

    Zuberi M.H. , '' (2006 ) 19 Pak. J. Pharm. Sci. : 231 -235 .

  • H. Amini, A. Ahmadiani, P. Gazerani, J. Chromatogr. B 824 (2005) 319–322.

    Gazerani P. , '' (2005 ) 824 J. Chromatogr. B : 319 -322 .

  • K. H. Yuen, K. K. Peh, J. Chromatogr. B 710 (1998) 243–246.

    Peh K. K. , '' (1998 ) 710 J. Chromatogr. B : 243 -246 .

  • M. Zhang, G.A. Moore, M. Lever, S.J. Gardiner, C.M. Kirkpatrick, E.J. Begg, J. Chromatogr. B 766 (2002) 175–179.

    Begg E.J. , '' (2002 ) 766 J. Chromatogr. B : 175 -179 .

  • O. Vesterqvist, F. Nabbie, B. Swanson, J. Chromatogr. B 716 (1998) 299–304.

    Swanson B. , '' (1998 ) 716 J. Chromatogr. B : 299 -304 .

  • A.R. Bonfigli, S. Manfrini, F. Gregorio, R. Testa, I. Testa, G. De Sio, G. Coppa, Ther. Drug Monit. 21 (1999) 330–334.

    Coppa G. , '' (1999 ) 21 Ther. Drug Monit. : 330 -334 .

  • X. Chen, Q. Gu, F. Qiu, D. Zhong, J. Chromatogr. B 802 (2004) 377–381.

    Zhong D. , '' (2004 ) 802 J. Chromatogr. B : 377 -381 .

  • H.N. Mistri, A.G. Jangid, P.S. Shrivastav, J. Pharm. Biomed. Anal. 45 (2007) 97–106.

    Shrivastav P.S. , '' (2007 ) 45 J. Pharm. Biomed. Anal : 97 -106 .

  • M.A. Marques, S. Soares Ade, O.W. Pinto, P.T. Barroso, D.P. Pinto, M. Ferreira-Filho, E. Werneck-Barroso, J. Chromatogr. B 852 (2007) 308–316.

    Werneck-Barroso E. , '' (2007 ) 852 J. Chromatogr. B : 308 -316 .

  • C.G. Ding, Z. Zhou, Q.H. Ge, X.J. Zhi, L.L. Ma, Biomed. Chromatogr. 21 (2007) 132–138.

    Ma L.L. , '' (2007 ) 21 Biomed. Chromatogr. : 132 -138 .

  • Y. Wang, Y. Tang, J. Gu, J.P. Fawcett, X. Bai, J. Chromatogr. B 808 (2004) 215–219.

    Bai X. , '' (2004 ) 808 J. Chromatogr. B : 215 -219 .

  • F. Collin, H. Khoury, D. Bonnefont-Rousselot, P. Tharond, A. Legrand, D. Jore, M. Gardas-Albert, J. Mass Spectrom. 39 (2004) 890–902.

    Gardas-Albert M. , '' (2004 ) 39 J. Mass Spectrom. : 890 -902 .

  • N. Koseki, H. Kawashita, M. Niina, Y. Nagae, N. Masuda, J. Pharm. Biomed. Anal. 36 (2005) 1063–1072.

    Masuda N. , '' (2005 ) 36 J. Pharm. Biomed. Anal. : 1063 -1072 .

  • A. Onal, Eur. J. Med. Chem. 44 (2009) 4998–5005.

    Onal A. , '' (2009 ) 44 Eur. J. Med. Chem. : 4998 -5005 .

  • A.R. Ali, Duraidi II, M.M. Saket, E.S. Abu-Nameh, J. AOAC Int. 92 (2009) 119–124.

    Abu-Nameh E.S. , '' (2009 ) 92 J. AOAC Int. : 119 -124 .

  • F. Al-Rimawi, Talanta 79 (2009) 1368–1371.

    Al-Rimawi F. , '' (2009 ) 79 Talanta : 1368 -1371 .

  • L.R. Wang, M.Z. Huang, S.H. Zhu, Zhejiang Da Xue Xue Bao Yi Xue Ban 34 (2005) 368–371.

    Zhu S.H. , '' (2005 ) 34 Zhejiang Da Xue Xue Bao Yi Xue Ban : 368 -371 .

  • K.M. Huttunen, J. Rautio, J. Leppanen, J. Epsalainen, P. Keski-Rahkonen, J. Pharm. Biomed. Anal. 50 (2009) 469–474.

    Keski-Rahkonen P. , '' (2009 ) 50 J. Pharm. Biomed. Anal. : 469 -474 .

  • S. Altinoz, D. Tekeli, J. Pharm. Biomed. Anal. 24 (2001) 507–515.

    Tekeli D. , '' (2001 ) 24 J. Pharm. Biomed. Anal. : 507 -515 .

  • K.H. Lehr, P. Damm, J. Chromatogr. 526 (1990) 497–505.

    Damm P. , '' (1990 ) 526 J. Chromatogr. : 497 -505 .

  • I.I. Salem, J. Idrees, J.I. Al Tamimi, J. Chromatogr. B 799 (2004) 103–109.

    Tamimi J.I. , '' (2004 ) 799 J. Chromatogr. B : 103 -109 .

  • C. Pistos, M. Koutsopoulou, I. Panderi, Biomed. Chromatogr. 19 (2005) 394–401.

    Panderi I. , '' (2005 ) 19 Biomed. Chromatogr. : 394 -401 .

  • S. El Deeb, U. Schepers, H. Watzig, J. Sep. Sci. 29 (2006) 1571–1577.

    Watzig H. , '' (2006 ) 29 J. Sep. Sci. : 1571 -1577 .

  • A. Karthik, G. Subramaniam, M.C. Rao, K. Bhat, A. Ranjithkumar, P. Musmade, M. Surulivelrajan, K. Karthikeyan, N. Udupa, Pak. J. Pharm. Sci. 21 (2008) 421–425.

    Udupa N. , '' (2008 ) 21 Pak. J. Pharm. Sci. : 421 -425 .

  • P. Kovarikova, J. Klimes, J. Dohnal, L. Tisovska, J. Pharm. Biomed. Anal. 36 (2004) 205–209.

    Tisovska L. , '' (2004 ) 36 J. Pharm. Biomed. Anal. : 205 -209 .

  • M.A. Khan, S. Sinha, S. Vartak, A. Bhartiya, S. Kumar, J. Pharm. Biomed. Anal. 39 (2005) 928–943.

    Kumar S. , '' (2005 ) 39 J. Pharm. Biomed. Anal. : 928 -943 .

  • G. Bansal, M. Singh, K.C. Jindal, S. Singh, J. Pharm. Biomed. Anal. 48 (2008) 788–795.

    Singh S. , '' (2008 ) 48 J. Pharm. Biomed. Anal. : 788 -795 .

  • B.L. Kolte, B.B. Raut, A.A. Deo, M.A. Bagool, D.B. Shinde, J. Chromatogr. Sci. 42 (2004) 70–73.

    Shinde D.B. , '' (2004 ) 42 J. Chromatogr. Sci. : 70 -73 .

  • R.T. Sane, S.N. Menon, S. Inamdar, M. Mote, A. Menezes, J. Planar Chromatogr. 17 (2004) 154–156.

    Menezes A. , '' (2004 ) 17 J. Planar Chromatogr. : 154 -156 .

  • D. Jain, S. Jain, D. Jain, M. Amin, J. Chromatogr. Sci. 46 (2008) 501–504.

    Amin M. , '' (2008 ) 46 J. Chromatogr. Sci. : 501 -504 .

  • R. Bhushan, D. Gupta, A. Jain, J. Planar Chromatogr. 19 (2006) 288–296.

    Jain A. , '' (2006 ) 19 J. Planar Chromatogr. : 288 -296 .

  • A. Gumieniczek, H. Hopkala, A. Berecka, D. Kowalczuk, J. Planar Chromatogr. 16 (2003) 271–275.

    Kowalczuk D. , '' (2003 ) 16 J. Planar Chromatogr. : 271 -275 .

  • A. Berecka, A. Gumieniczek, H. Hopkala, J. Planar Chromatogr. 18 (2005) 61–66.

    Hopkala H. , '' (2005 ) 18 J. Planar Chromatogr. : 61 -66 .

  • A. Gumieniczek, H. Hopkala, A. Berecka, J. Liq. Chromatogr. Relat. Technol. 27 (2004) 2057–2070.

    Berecka A. , '' (2004 ) 27 J. Liq. Chromatogr. Relat. Technol. : 2057 -2070 .

  • PART II: Validation of analytical procedure: methodology Q2B, ICH Harmonized Tripartite Guidelines, Geneva, 1996.

  • International Conference on Harmonization, Validation of Analytical Procedures: Methodology, Federal Register, Geneva, 1996.

    '', in Validation of Analytical Procedures: Methodology , (1996 ) -.

  • Collapse
  • Expand

To see the editorial board, please visit the website of Springer Nature.

Manuscript Submission: HERE

For subscription options, please visit the website of Springer Nature.

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Dec 2023 30 0 0
Jan 2024 28 3 0
Feb 2024 38 0 0
Mar 2024 11 0 0
Apr 2024 18 0 0
May 2024 18 0 0
Jun 2024 7 0 0