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Susheel Varghese College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences Department of Pharmaceutical Analysis Coimbatore 641044 Tamil Nadu India

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P. Vasanthi College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences Department of Pharmaceutical Analysis Coimbatore 641044 Tamil Nadu India

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Thengungal Ravi College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences Department of Pharmaceutical Analysis Coimbatore 641044 Tamil Nadu India

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This paper describes a validated high-performance thin-layer chromatography (HPTLC) method for the simultaneous determination of ivermectin (IVM) and albendazole (ALB) in combined tablet dosage forms. The HPTLC separation was carried out on aluminum-backed silica gel 60 F254 layers using toluene-ethyl acetateglacial acetic acid, 6:4:0.5 (ν/ν/ν), as the mobile phase. Quantification was achieved with UV detection at 247 nm. The developed analytical method was validated according to International Conference on Harmonization guidelines in terms of parameters such as accuracy, precision, linearity, and specificity. Good linearity was observed in the concentrations ranging from 0.12 to 0.54 and 8 to 36 μg per band for IVM and ALB, respectively. The recovery study results ranged from 98% to 101% for IVM and ALB, showing the accuracy of the method. The method was successfully applied for the analysis of the drugs in laboratory-prepared mixtures and commercial tablets. The developed method is simple, precise, and sensitive, and applicable for the simultaneous determination of IVM and ALB in pure powder and tablets.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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