JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 24: Issue 5
Simultaneous quantification of two bioactive lupane triterpenoids from Diospyros melanoxylon stem bark
Authors:
Kedar Rout North Orissa University P.G. Department of Chemistry Sriramchandra Vihar, Baripada, Mayurbhanja 757003 Orissa India

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Rajesh Singh North Orissa University P.G. Department of Chemistry Sriramchandra Vihar, Baripada, Mayurbhanja 757003 Orissa India

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Sagar Mishra Utkal University Pharmacognosy and Phytochemistry Division, University Department of Pharmaceutical Sciences Vani Vihar, Bhubaneswar 751004 Orissa India

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Pages:
376–380
Online Publication Date:
21 Oct 2011
Publication Date:
01 Oct 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.24.2011.5.3
Keywords:
High-performance thin-layer chromatography (HPTLC); Densitometry; TLC; Lupeol; Betulin; Diospyros melanoxylon
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A high-performance thin-layer chromatography (HPTLC) method has been developed and validated for the quantification of two bioactive lupane triterpenoids, namely, lupeol and betulin from Diospyros melanoxyon stem bark. Chromatographic separation was achieved on aluminium foil-backed HPTLC plates using ethyl acetate-hexane (1.8:8.2, v/v) as mobile phase. The compounds were quantified at their wave length of maximum absorbance in the range of 100–500 ng per spot. The instrumental precision was 0.82% and 1.07% (CV) and the repeatability of the method was 1.33% and 1.17% (CV), respectively, for lupeol and betulin. The minimum detectable amount was found to be 40 and 50 ng per spot for lupeol and betulin, respectively. The linear regression analysis data for the calibration plots showed a good linear relationship with r2 = 0.9996 for lupeol and 0.9997 for betulin. The method was validated for precision, recovery, and repeatability as per the International Conference on Harmonization guidelines. The developed HPTLC method is very accurate and precise, and has been successfully applied for the assay of these bioactive molecules in D. melanoxylon stem bark.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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