A high-performance thin-layer chromatography (HPTLC) method has been developed and validated for the quantification of two bioactive lupane triterpenoids, namely, lupeol and betulin from Diospyros melanoxyon stem bark. Chromatographic separation was achieved on aluminium foil-backed HPTLC plates using ethyl acetate-hexane (1.8:8.2, v/v) as mobile phase. The compounds were quantified at their wave length of maximum absorbance in the range of 100–500 ng per spot. The instrumental precision was 0.82% and 1.07% (CV) and the repeatability of the method was 1.33% and 1.17% (CV), respectively, for lupeol and betulin. The minimum detectable amount was found to be 40 and 50 ng per spot for lupeol and betulin, respectively. The linear regression analysis data for the calibration plots showed a good linear relationship with r2 = 0.9996 for lupeol and 0.9997 for betulin. The method was validated for precision, recovery, and repeatability as per the International Conference on Harmonization guidelines. The developed HPTLC method is very accurate and precise, and has been successfully applied for the assay of these bioactive molecules in D. melanoxylon stem bark.
G. Watt, A Dictionary of the Economic Products of India, Vol. III, Cosmopublication, New Delhi, 1952, p. 136.
Watt G., '', in A Dictionary of the Economic Products of India, Vol. III, (1952) -.
Watt G.A Dictionary of the Economic Products of India, Vol. III1952)| false
B.N. Sastry, The Wealth of India, Raw Materials, Vol. III, CSIR, New Delhi, 1952, p.76.
Sastry B.N., '', in The Wealth of India, Raw Materials, Vol. III, (1952) -.
Sastry B.N.The Wealth of India, Raw Materials, Vol. III1952)| false
R.K. Gupta, P.S. Rao, Proc. Indian Acad. Sci. A 60 (1964) 36–41.
Rao P.S., '' (1964) 60Proc. Indian Acad. Sci. A: 36-41.
Rao P.S.Proc. Indian Acad. Sci. A1964603641)| false
Validation of analytical procedure: methodology Q2 (R1), International Conference on Harmonization (ICH), International Federation of Pharmaceutical Manufacturers and Associations (IFPMA), Geneva, 1996.
K.K. Rout, S.K. Mishra, J. Planar Chromatogr. 22 (2009) 127–131.