JPC - Journal of Planar Chromatography - Modern TLC
Volume/Issue:
Volume 24: Issue 5
TLC-densitometric method development and validation for the quantification of nicotine in tobacco smoked, sniffing, dipping, and chewing products
Authors:
Syed MusharrafUniversity of Karachi H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences Karachi 75270 Pakistan

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Muhammad ShoaibBahauddin Zakariya University (B.Z.U.) Department of Chemistry Multan 60800 Pakistan

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Najam-ul-HaqBahauddin Zakariya University (B.Z.U.) Department of Chemistry Multan 60800 Pakistan

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Pages:
381–387
Online Publication Date:
21 Oct 2011
Publication Date:
01 Oct 2011
Article Category:
Research Article
DOI:
https://doi.org/10.1556/jpc.24.2011.5.4
Keywords:
Nicotine quantification; Nicotiana tabacum ; Tobacco products; TLC-densitometry
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A sensitive, selective, precise, and robust densitometric method for the analysis of nicotine (drug component of Nicotiana tabacum) in various tobacco products (TPS), including smoked (cigarette and beedi), sniffing (sniffing niswar), dipping (niswar), and chewing (gutka) tobacco brands, was developed and validated as per the ICH guideline. TLC glass plates, precoated with silica gel 60F-254, were used for the analysis. Densitometric analysis of nicotine was carried out in the absorbance mode at 262 nm. The mobile phase consisted of petroleum ether-acetone-diethylamine (7.6:2:0.4) v/v, and gave a sharp and symmetrical nicotine peak (RF = 0.57 ± 0.005) under saturated environment. Linear regression data (LRD) for the calibration curve showed a good linearity with r = 0.997 ± 0.0013. The method was validated for precision, recovery, and robustness. The limits of detection (LOD) and quantitation (LOQ) were found to be 3.15 and 9.54 ng per spot, respectively. Statistical analysis indicated that the method is reproducible and selective for the estimation of nicotine in various TPS.

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Cover JPC - Journal of Planar Chromatography - Modern TLC
JPC - Journal of Planar Chromatography - Modern TLC
Print ISSN:
0933-4173
Online ISSN:
1789-0993

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation		 1988
Volumes
per Year		 1
Issues
per Year		 6
Founder Akadémiai Kiadó
Founder's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address		 H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher		 Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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