View More View Less
  • 1 University of Karachi H.E.J. Research Institute of Chemistry, International Center for Chemical and Biological Sciences Karachi 75270 Pakistan
  • | 2 Bahauddin Zakariya University (B.Z.U.) Department of Chemistry Multan 60800 Pakistan
Restricted access

A sensitive, selective, precise, and robust densitometric method for the analysis of nicotine (drug component of Nicotiana tabacum) in various tobacco products (TPS), including smoked (cigarette and beedi), sniffing (sniffing niswar), dipping (niswar), and chewing (gutka) tobacco brands, was developed and validated as per the ICH guideline. TLC glass plates, precoated with silica gel 60F-254, were used for the analysis. Densitometric analysis of nicotine was carried out in the absorbance mode at 262 nm. The mobile phase consisted of petroleum ether-acetone-diethylamine (7.6:2:0.4) v/v, and gave a sharp and symmetrical nicotine peak (RF = 0.57 ± 0.005) under saturated environment. Linear regression data (LRD) for the calibration curve showed a good linearity with r = 0.997 ± 0.0013. The method was validated for precision, recovery, and robustness. The limits of detection (LOD) and quantitation (LOQ) were found to be 3.15 and 9.54 ng per spot, respectively. Statistical analysis indicated that the method is reproducible and selective for the estimation of nicotine in various TPS.

  • World Health Organization (WHO), Tobacco or Health: A Global Status Report, Geneva, 1997.

  • The World Health Report, World Health Organization, Geneva, 2006.

  • K. Ahmad, F. Jafary, I. Jehan, J. Hatcher, A.Q. Khan, N. Chaturvedi, T.H. Jafar, Eur. J. Cardiovasc. Prev. Rehabil. 12 (2005) 203–208.

    Jafar T.H. , '' (2005 ) 12 Eur. J. Cardiovasc. Prev. Rehabil. : 203 -208.

  • J.C. Stavrides, Free Radic. Biol. Med. 41 (2006) 1017–1030.

    Stavrides J.C. , '' (2006 ) 41 Free Radic. Biol. Med. : 1017 -1030.

  • M. Akbartabartoori, J.L. Me, C.R. Hankey, Eur. J. Cardiovasc. Prev. Rehabil. 13 (2006) 938–946.

    Hankey C.R. , '' (2006 ) 13 Eur. J. Cardiovasc. Prev. Rehabil. : 938 -946.

  • I.P. Stolerman, M.J. Jarvis, Psychopharmacology 117 (1995) 2–10.

    Jarvis M.J. , '' (1995 ) 117 Psychopharmacology : 2 -10.

  • F.E. Pontieri, G. Tanda, F. Orzi, G.D. Chiara, Nature 382 (1996) 255–257.

    Chiara G.D. , '' (1996 ) 382 Nature : 255 -257.

  • E.M. Pich, S.R. Pagliusi, M. Tessari, D. Talabot-Ayer, R.H. van Huijsduijnen, C. Chiamuler, Science 275 (1997) 83–86.

    Chiamuler C. , '' (1997 ) 275 Science : 83 -86.

  • J.G. Nel, S. Afr. J. Plant Soil 5 (1988) 40–41.

    Nel J.G. , '' (1988 ) 5 S. Afr. J. Plant Soil : 40 -41.

  • K. Tyrpiebosz, A. Chrüoeciewicz, M. Ciolecka, T. Wielkoszyński, B. Janoszka, D. Bodzek, Acta Chromatogr. 13 (2003) 154–160.

    Bodzek D. , '' (2003 ) 13 Acta Chromatogr. : 154 -160.

  • R.D. Handral, M.N. Ravishankara, S.A. Shah, Indian J. Pharm. Sci. 63 (2001) 250–253.

    Shah S.A. , '' (2001 ) 63 Indian J. Pharm. Sci. : 250 -253.

  • ICH, Q2A validation of analytical procedure: methodology, in: Proceedings of the International Conference on Harmonization, Geneva, October 1994.

  • ICH, Q2B validation of analytical procedure: methodology, in: Proceedings of the International Conference on Harmonization, Geneva, March 1996.

  • ICH guidance on analytical method validation in: Proceedings of the International Convention on Quality for the Pharmaceutical Industry, Toronto, Canada, September 2002.

  • M.J. Binette, P. Lafontaine, M. Vanier, L.K. Ng, J. Agric. Food Chem. 57 (2009) 1151–1155.

    Ng L.K. , '' (2009 ) 57 J. Agric. Food Chem. : 1151 -1155.

All Time Past Year Past 30 Days
Abstract Views 36 36 2
Full Text Views 10 7 0
PDF Downloads 3 1 0