A sensitive, selective, precise, and robust densitometric method for the analysis of nicotine (drug component of Nicotiana tabacum) in various tobacco products (TPS), including smoked (cigarette and beedi), sniffing (sniffing niswar), dipping (niswar), and chewing (gutka) tobacco brands, was developed and validated as per the ICH guideline. TLC glass plates, precoated with silica gel 60F-254, were used for the analysis. Densitometric analysis of nicotine was carried out in the absorbance mode at 262 nm. The mobile phase consisted of petroleum ether-acetone-diethylamine (7.6:2:0.4) v/v, and gave a sharp and symmetrical nicotine peak (RF = 0.57 ± 0.005) under saturated environment. Linear regression data (LRD) for the calibration curve showed a good linearity with r = 0.997 ± 0.0013. The method was validated for precision, recovery, and robustness. The limits of detection (LOD) and quantitation (LOQ) were found to be 3.15 and 9.54 ng per spot, respectively. Statistical analysis indicated that the method is reproducible and selective for the estimation of nicotine in various TPS.
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K. Ahmad, F. Jafary, I. Jehan, J. Hatcher, A.Q. Khan, N. Chaturvedi, T.H. Jafar, Eur. J. Cardiovasc. Prev. Rehabil. 12 (2005) 203–208.
Jafar T.H., '' (2005) 12Eur. J. Cardiovasc. Prev. Rehabil.: 203-208.
Jafar T.H.Eur. J. Cardiovasc. Prev. Rehabil.200512203208)| false