For the purpose of qualitative and quantitative analysis of flavonoids from eight species of Indocalamus, Indocalamus latifolius (Keng) McClure, I. herklotsii McClure, I. decorus Q. H. Dai, I. barbatus McClure, I. sinicus (Hance) Nakai, I. longiauritus Hand.—Mazz, I. guangdongensis H. R. Zhao et Y. L. Yang, I. pedalis (Keng) Keng f., a simple, efficient, accurate and low-cost method was constructed. Six flavonoids, vitexin, isovitexin, orientin, isoorientin, quercetin and tricin, were selected as marker flavonoids. The multistage development was performed in Automated Multiple Development (AMD2) and solvents in different ratios were used as mobile phase. The developed plates were scanned by TLC Scanner3. The components separated well and RF values of the six marker flavonoids were 0.22, 0.31, 0.38, 0.45, 0.57, and 0.88, respectively. The method was validated for precision, accuracy, selectivity and repeatability, which were expressed as coefficient of variation (CV [%]). The concentrations and kinds of flavonoids were significantly different in these six species of Indocalamus. The concentration of total flavonoids in the dry leaves was in the range of 0.11–0.40%, and the decreasing order of concentration was I. barbatus, I. sinicus, I. longiauritus, I. guangdongensis, I. decorus, I. pedalis, I. latifolius, I. herklotsii; the concentration of total flavonoids in I. barbatus was the maximum among these eight species of Indocalamus, and they would be more useful in extracting natural bamboo flavonoids.
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