Quantitative evaluation of phytochemical diversity in Adhatoda vasica Nees populations from five different ecogeographical regions was performed using a highly sensitive, robust and economic high performance thin layer chromatography (HPTLC) procedure. The method was validated for specificity, precision and accuracy as per the guidelines affirmed by International Conference on Harmonization of technical requirements for registration of pharmaceuticals for human use (ICH). The findings support the existence of distinct natural chemotypes within the species. Two out of the five regions had about three times higher mean vasicine content than the remaining three regions. The comparative phytochemical fingerprint profiles were quite similar except for the concentration variation seen for different alkaloids. As the quinazoline alkaloids are the biologically active compounds in A. vasica, the samples that accumulate high levels of alkaloids seem to be promising for further propagation.
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