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  • 1 University of Łódź Department of Instrumental Analysis Pomorska 163 90-236 Łódź Poland
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The paper presents the application of post-chromatographic iodine-azide reaction for the determination of three thiouracils (6-benzyl-, 6-methyl-, and 6-propyl-2-thiouracil) in high-performance thin-layer chromatography (HPTLC). The HPTLC plates developed by methanol were sprayed with a freshly prepared mixture of sodium azide, potassium iodide, and starch solution adjusted to pH 5.5, and exposed to iodine vapour for 5 s. Because of the induction properties of the C-S and C=S bonds of thiouracil molecule in the iodine-azide reaction, the spots became visible as white spots on a violet-brown background.Scanning of the HPTLC plates was performed on a PC scanner and analysed by TLSee software. Determination range was 7–16 pmol per spot, 80–160 nmol per mL of urine, or 133–266 nmol per 1 mL of serum.

  • R. Wilson, M. Choppra, H. Bradley, J.H. McKillop, Clin. Endocrinol. 77 (1990) 149–153.

    McKillop J.H. , '' (1990 ) 77 Clin. Endocrinol. : 149 -153.

  • M. Lazzarin, L. Segattini, F. Benati, Recenti Prog. Med. 84 (1993) 698–708.

    Benati F. , '' (1993 ) 84 Recenti Prog. Med. : 698 -708.

  • W.R. Griswold, S.A. Mendoza, W. Johnston, S. Nichols, Western J. Med. 128 (1978) 543–546.

    Nichols S. , '' (1978 ) 128 Western J. Med. : 543 -546.

  • P. Batjoens, H.F. De Brabander, L. T’Kindt, Anal. Chim. Acta 275 (1993) 335–340.

    T’Kindt L. , '' (1993 ) 275 Anal. Chim. Acta : 335 -340.

  • European Community Directive 86/649 Off. J. Eur. Commun. 36 (1986) Document Number L275.

  • G. Pinel, E. Bichon, K. Pouponneaus, D. Maume, F. André, B. Bizec, J. Chromatogr. A 1085 (2005) 247–252.

    Bizec B. , '' (2005 ) 1085 J. Chromatogr. A : 247 -252.

  • Q.H. Zou, Y. Liu, M.X. Xie, J. Han, L. Zhang, Anal. Chim. Acta 551 (2005) 184–191.

    Zhang L. , '' (2005 ) 551 Anal. Chim. Acta : 184 -191.

  • P. Batjoens, H.F. De Brabander, K. De Wasch, J. Chromatogr. A 750 (1996) 127–132.

    Wasch K. , '' (1996 ) 750 J. Chromatogr. A : 127 -132.

  • H.G. Giles, R. Miller, E.M. Sellers, J. Pharm. Sci. 68 (1979) 1459–1460.

    Sellers E.M. , '' (1979 ) 68 J. Pharm. Sci. : 1459 -1460.

  • H.P. Ringhand, W.A. Ritschel, J. Pharm. Sci. 68 (1979) 1461–1463.

    Ritschel W.A. , '' (1979 ) 68 J. Pharm. Sci. : 1461 -1463.

  • M.T. Rosseel, R.A. Lefebvre, J. Chromatogr. 507 (1990) 247–251.

    Lefebvre R.A. , '' (1990 ) 507 J. Chromatogr. : 247 -251.

  • G.R. Cannell, J.P. Williams, A.S. Yap, R.H. Mortimer, J. Chromatogr. 564 (1991) 310–314.

    Mortimer R.H. , '' (1991 ) 564 J. Chromatogr. : 310 -314.

  • G. Moretti, P. Betto, P. Cammarata, F. Fracassi, M. Giambendetti, J. Chromatogr. 616 (1993) 291–296.

    Giambendetti M. , '' (1993 ) 616 J. Chromatogr. : 291 -296.

  • L. Kabanda, C. De Muynck, R.A. Lefebvre, J.P. Remon, J. Liq. Chromatogr. 17 (1994) 2069–2083.

    Remon J.P. , '' (1994 ) 17 J. Liq. Chromatogr. : 2069 -2083.

  • M.-F. Pochard, M. Karageorgis, M. Chevalier, J. Chromatogr. 298 (1984) 183–188.

    Chevalier M. , '' (1984 ) 298 J. Chromatogr. : 183 -188.

  • C.G. Duarte, A.C.M. Polizello, A.I. Assis-Pandochi, A.C.C. Spadaro, J. Pharm. Biomed. Anal. 23 (2000) 237–241.

    Spadaro A.C.C. , '' (2000 ) 23 J. Pharm. Biomed. Anal. : 237 -241.

  • V.M. Obeidat, Acta Pharm. Sci. 48 (2006) 205–211.

    Obeidat V.M. , '' (2006 ) 48 Acta Pharm. Sci. : 205 -211.

  • Y. Wei, Z.J. Zhang, Y.T. Zhang, Y.H. Sun, J. Chromatogr. B 854 (2007) 239–244.

    Sun Y.H. , '' (2007 ) 854 J. Chromatogr. B : 239 -244.

  • R.K. Buick, C. Barry, I.M. Traynor, W.J. McCaughey, Ch.T. Elliott, J. Chromatogr. B 720 (1998) 71–79.

    Elliott Ch.T. , '' (1998 ) 720 J. Chromatogr. B : 71 -79.

  • H. Hooijerink, W.G. De Ruig, J. Chromatogr. 394 (1987) 403–407.

    Ruig W.G. , '' (1987 ) 394 J. Chromatogr. : 403 -407.

  • K. De Wasch, H.F. Brabander, S. Impens, M. Vandewiele, D. Courtheyn, J. Chromatogr. A 912 (2001) 311–317.

    Courtheyn D. , '' (2001 ) 912 J. Chromatogr. A : 311 -317.

  • B. le Bizec, F. Monteau, D. Maume, M.-P. Montrade, C. Gade, F. André, Anal. Chim. Acta 340 (1997) 201–208.

    André F. , '' (1997 ) 340 Anal. Chim. Acta : 201 -208.

  • T. Ohkubo, T. Uno, K. Sugawara, J. Liq. Chromatogr. Relat. Technol. 14 (1991) 2745–2753.

    Sugawara K. , '' (1991 ) 14 J. Liq. Chromatogr. Relat. Technol. : 2745 -2753.

  • D.G. Watson, C.D. Bates, G.G. Skellern, R. Mairs, S. Martin, C.J.W. Brooks, Rapid Commun. Mass Spectrom. 5 (2005) 141–142.

    Brooks C.J.W. , '' (2005 ) 5 Rapid Commun. Mass Spectrom. : 141 -142.

  • K. Štulik, V. Pacáková, J. Chromatogr. B 273 (1983) 77–86.

    Pacáková V. , '' (1983 ) 273 J. Chromatogr. B : 77 -86.

  • P. Mitra, A.T. Riga, K.S. Alexander, J. Liq. Chromatogr. Relat. Technol. 27 (2004) 549–558.

    Alexander K.S. , '' (2004 ) 27 J. Liq. Chromatogr. Relat. Technol. : 549 -558.

  • R. Zakrzewski, W. Ciesielski, J. Chromatogr. B 784 (2003) 283–290.

    Ciesielski W. , '' (2003 ) 784 J. Chromatogr. B : 283 -290.

  • K. De Wasch, H.F. de Brabander, L.A. van Ginkel, A. Spaan, S.S. Sterk, H.D. Meiring, J. Chromatogr. A 819 (1998) 99–111.

    Meiring H.D. , '' (1998 ) 819 J. Chromatogr. A : 99 -111.

  • G. Moretti, M. Amici, P. Cammarata, F. Fracassi, J. Chromatogr. A 442 (1988) 459–463.

    Fracassi F. , '' (1988 ) 442 J. Chromatogr. A : 459 -463.

  • R. Zakrzewski, W. Ciesielski, Trends Chromatogr. 3 (2007) 1–10.

    Ciesielski W. , '' (2007 ) 3 Trends Chromatogr. : 1 -10.

  • J. Kossakowski, T. Kłopocki, B. Żbikowski, Farm. Pol. 7 (1972) 707–710.

    Żbikowski B. , '' (1972 ) 7 Farm. Pol. : 707 -710.

  • J. Kurzawa, Quim. Anal. 4 (1985) 117–128.

    Kurzawa J. , '' (1985 ) 4 Quim. Anal. : 117 -128.

  • J. Kurzawa, Chem. Anal. 32 (1987) 875–890.

    Kurzawa J. , '' (1987 ) 32 Chem. Anal. : 875 -890.

  • J. Kurzawa, A. Wioeniewska, Chem. Anal. 50 (2005) 1043–1050.

    Wioeniewska A. , '' (2005 ) 50 Chem. Anal. : 1043 -1050.

  • J. Kurzawa, A. Wioeniewska, K. Janowicz, Anal. Chem. Acta 567 (2006) 286–292.

    Janowicz K. , '' (2006 ) 567 Anal. Chem. Acta : 286 -292.

  • R. Zakrzewski, J. Chromatogr. B 869 (2008) 67–74.

    Zakrzewski R. , '' (2008 ) 869 J. Chromatogr. B : 67 -74.

  • R. Zakrzewski, J. Sep. Sci. 31 (2008) 2199–2205.

    Zakrzewski R. , '' (2008 ) 31 J. Sep. Sci. : 2199 -2205.

  • R. Zakrzewski, J. Pharm. Biomed. Anal. 48 (2008) 145–150.

    Zakrzewski R. , '' (2008 ) 48 J. Pharm. Biomed. Anal. : 145 -150.

  • R. Zakrzewski, Arch. Pharm. 31 (2008) 1622–1630.

    Zakrzewski R. , '' (2008 ) 31 Arch. Pharm. : 1622 -1630.

  • R. Zakrzewski, J. Liq. Chromatogr. Relat. Technol. 32 (2009) 383–398.

    Zakrzewski R. , '' (2009 ) 32 J. Liq. Chromatogr. Relat. Technol. : 383 -398.

  • R. Zakrzewski, Anal. Lett. 42 (2009) 1157–1163.

    Zakrzewski R. , '' (2009 ) 42 Anal. Lett. : 1157 -1163.

  • R. Zakrzewski, J. Liq. Chromatogr. Relat. Technol. 32 (2009) 2499–2511.

    Zakrzewski R. , '' (2009 ) 32 J. Liq. Chromatogr. Relat. Technol. : 2499 -2511.

  • R. Zakrzewski, W. Ciesielski, in: J. Cazes (ed.) Encyclopedia of Chromatography, 3rd edn., Taylor and Francis Group, New York, 2010, 1: 2, 1226–1233.

    Ciesielski W. , '', in Encyclopedia of Chromatography , (2010 ) -.

  • R. Zakrzewski, W. Ciesielski, J. Liq. Chromatogr. Relat. Technol. 31 (2008) 2657–2672.

    Ciesielski W. , '' (2008 ) 31 J. Liq. Chromatogr. Relat. Technol. : 2657 -2672.

  • J. Kurzawa, Polish J. Chem. 58 (1984) 373–376.

    Kurzawa J. , '' (1984 ) 58 Polish J. Chem. : 373 -376.

  • W. Ciesielski, Z.H. Kudzin, P. Kiełbasińki, Talanta 41 (1994) 1493–1498.

    Kiełbasińki P. , '' (1994 ) 41 Talanta : 1493 -1498.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
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