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  • 1 Laboratory for Food Chemistry National Institute of Chemistry Hajdrihova 19 SI-1001 Ljubljana Slovenia
  • | 2 University of Zagreb Laboratory of Analytical Chemistry, Faculty of Chemical Engineering and Technology Marulićev trg 19 10000 Zagreb Croatia
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Validation of a method for the quantification of (−)-epicatechin and procyanidin B2 in chocolates is reported. After a simple preparation of the chocolate test solution, thin-layer chromatography (TLC) on high-performance thin-layer chromatographic (HPTLC) cellulose plates with n-propanol-water-acetic acid (20:80:1, ν/ν) and derivatization with 4-dimethylaminocinnamaldehyde (DMACA) were performed. Densitometry at 655 nm enabled accurate quantification of both analytes in chocolates. The milk chocolate sample which served for precision and recovery experiments contained 13.0 mg/100 g of (−)-epicatechin (relative standard deviation (RSD)) 5.8%, n = 6) and 13.2 mg/100 g of procyanidin B2 (RSD 4.2%, n = 6). Usable polynomial curves were 2–30 ng for (−)-epicatechin and 4–60 ng for procyanidin B2. The validated TLC method can be applied for routine quality control of the two major polyphenolic compounds in different chocolate samples.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
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1988
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1
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6
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Springer Nature Switzerland AG
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ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

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