A selective high-performance thin-layer chromatographic (HPTLC) method for the quantification of vitamin D3 was developed and validated for the analysis of fish oils. The fish oil samples were applied onto the HPTLC plates after saponification and purification.HPTLC of fish oils was performed on Kieselgel 60 F254 (10 cm × 10 cm) plates with chloroform-ether 9:1 (ν/ν) as the mobile phase. Quantitative evaluation of the plate was performed at 280 nm without derivatization. The assay provides good accuracy, reproducibility, and selectivity for the quantitative analysis of vitamin D3. The calibration plots were linear in the range 200–1000 ng per spot. The technique was used for the estimation of the vitamin D3 content of fish oils, lipid food supplements and drugs containing vitamin D3.
Institute of Medicine (IOM), Dietary Reference Intakes for Calcium, Phosphorus, Magnesium, Vitamin D, and Fluoride, National Academy Press, Washington, D.C., 1997.
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