Enicostemma littorale (Chota-Chirayata or Mamejava) is a glabrous perennial herb belonging to the family Gentianaceae. This plant has been widely used in traditional system of medicines for the treatment of diabetes, malaria, and fever. Its anti-inflammatory, antioxidant, hypoglycemic, hepatoprotective, antihyperlipidaemic antiedematogenic, and anticancer activities have been reported. Ahigh-performance thin-layer chromatography (HPTLC) method has therefore been established for the simultaneous quantification of three biomarkers, namely, isoswertisin-5-O-β-d-glucoside, swertiamarin, and swertisin in the powder of E. littorale Blume. Chromatographic separation was performed on silica gel plates with ethyl acetatemethanol-water 8.0:1.0:0.5 (ν/ν) as mobile phase. Detection and quantitation were performed by densitometry with a deuterium lamp at 287 nm. The responses to reference standards were linear in the concentration range 25–75 μg mL−1 for isoswertisin-5-O-β-d-glucoside, 200–600 μg mL−1 for swertiamarin, and 100–300 μg mL−1 for swertisin. The relative standard deviation (RSD) for instrumental precision, intra-assay precision, and intermediate precision was <2%. Swertiamarin was present at high concentration and isoswertisin-5-O-β-d-glucoside at low concentration in the whole-plant powder. The accuracy of the method was determined by the measurement of recovery at three different concentrations. The average recovery was 99.88% for isoswertisin-5-O-β-d-glucoside, 99.64% for swertiamarin, and 99.10% for swertisin. The proposed HPTLC method was found to be simple, precise, selective, sensitive, and accurate for routine quality control of the whole-plant powder of E. littorale Blume.
P. Bhandari, N. Kumar, A.P. Gupta, B. Singh, V.K. Kaul, Chromatographia 64 (2006) 599–602.
Kaul V.K., '' (2006) 64Chromatographia: 599-602.
Kaul V.K.Chromatographia200664599602)| false
J. Sadique, T. Chandra, V. Thenmozhi, V. Elango, Biochem. Med. Metab. Biol. 37 (1987) 167–176.