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  • 1 SVKM’s NMIMS School of Pharmacy & Technology Management Vile Parle (W) Mumbai 400056 India
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Enicostemma littorale (Chota-Chirayata or Mamejava) is a glabrous perennial herb belonging to the family Gentianaceae. This plant has been widely used in traditional system of medicines for the treatment of diabetes, malaria, and fever. Its anti-inflammatory, antioxidant, hypoglycemic, hepatoprotective, antihyperlipidaemic antiedematogenic, and anticancer activities have been reported. Ahigh-performance thin-layer chromatography (HPTLC) method has therefore been established for the simultaneous quantification of three biomarkers, namely, isoswertisin-5-O-β-d-glucoside, swertiamarin, and swertisin in the powder of E. littorale Blume. Chromatographic separation was performed on silica gel plates with ethyl acetatemethanol-water 8.0:1.0:0.5 (ν/ν) as mobile phase. Detection and quantitation were performed by densitometry with a deuterium lamp at 287 nm. The responses to reference standards were linear in the concentration range 25–75 μg mL−1 for isoswertisin-5-O-β-d-glucoside, 200–600 μg mL−1 for swertiamarin, and 100–300 μg mL−1 for swertisin. The relative standard deviation (RSD) for instrumental precision, intra-assay precision, and intermediate precision was <2%. Swertiamarin was present at high concentration and isoswertisin-5-O-β-d-glucoside at low concentration in the whole-plant powder. The accuracy of the method was determined by the measurement of recovery at three different concentrations. The average recovery was 99.88% for isoswertisin-5-O-β-d-glucoside, 99.64% for swertiamarin, and 99.10% for swertisin. The proposed HPTLC method was found to be simple, precise, selective, sensitive, and accurate for routine quality control of the whole-plant powder of E. littorale Blume.

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Journal of Planar Chromatography - Modern TLC
Language English
Size A4
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1988
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ISSN 0933-4173 (Print)
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