View More View Less
  • 1 (CSIR) Palampur Natural Plant Products Division, Institute of Himalayan Bioresource Technology Himachal Pradesh 176 061 India
Restricted access

A reversed-phase high-performance thin-layer chromatography (RP-HPTLC) method was developed for the determination of flavonoids (quercetin and kaempferol) and biflavonoids (sciadopitysin, ginkgetin, and bilobetin) in the aqueous methanolic extract of Ginkgo biloba. There have been a number of reports on the quantification of flavonoids in G. biloba using different analytical techniques, but the reported methods are not suitable for routine analysis and also for the simultaneous quantification of flavonoids and biflavonoids in G. biloba. The method employed here used precoated plates of silica gel 60F254 as the stationary phase with dual-run acetonitrile-water-methanol-formic acid (20:20:1:0.005, ν/ν/ν/ν) and acetonitrile-water-methanol-formic acid (20:17:1:0.005, ν/ν/ν/ν) as mobile phases with densitometric determination of flavonoids and biflavonoids at 254 nm in reflection/absorption mode. The linear regression analysis data for the calibration plots showed a linear relationship (r2 from 0.9706 to 0.9990). The method was validated for accuracy, precision, and robustness. The limits of detection (LOD) and quantification (LOQ) were in the range of 0.12–0.37 μg and 0.60–1.85 μg, respectively, for the analytes. The method is reproducible and convenient for quantitative analysis of these compounds in the leaves of G. biloba. This is the first report of simultaneous densitometry quantification of major flavonoids and biflavonoids in G. biloba using the newly developed and validated RP-HPTLC method.

  • A.Y. Leung, Encyclopedia of Common Natural Ingredients used in Food, Drugs and Cosmetics, John Wiley & Sons, New York, 1996.

    Leung A.Y. , '', in Encyclopedia of Common Natural Ingredients used in Food, Drugs and Cosmetics , (1996 ) -.

  • T.A. Van Beek, Ginkgo biloba, 12th edn., Harwood Academic Publishers, Amsterdam, 2000.

    Beek T.A. , '', in Ginkgo biloba , (2000 ) -.

  • B. Singh, P. Kaur, G. Chand, R.D. Singh, P.S. Ahuja, Fitoterapia 79 (2008) 401–418.

    Ahuja P.S. , '' (2008 ) 79 Fitoterapia : 401 -418.

  • P. Bhandari, N. Kumar, A.P. Gupta, B. Singh, V.K. Kaul, J. Sep. Sci. 30 (2007) 2092–2096.

    Kaul V.K. , '' (2007 ) 30 J. Sep. Sci. : 2092 -2096.

  • P. Kaur, A. Chaudhary, B. Singh, G. Chand, J. Pharm. Biomed. Anal. 50 (2009) 1060–1064.

    Chand G. , '' (2009 ) 50 J. Pharm. Biomed. Anal. : 1060 -1064.

  • N. Singh, A.P. Gupta, B. Singh, V.K. Kaul, J. Liq. Chromatogr. Relat. Technol. 28 (2005) 1679–1691.

    Kaul V.K. , '' (2005 ) 28 J. Liq. Chromatogr. Relat. Technol. : 1679 -1691.

  • N. Mishra, A.P. Gupta, B. Singh, V.K. Kaul, J. Liq. Chromatogr. Relat. Technol. 28 (2005) 665–679.

    Kaul V.K. , '' (2005 ) 28 J. Liq. Chromatogr. Relat. Technol. : 665 -679.

  • P. Bhandari, A.P. Gupta, B. Singh, V.K. Kaul, J. Sep. Sci. 28 (2005) 2288–2292.

    Kaul V.K. , '' (2005 ) 28 J. Sep. Sci. : 2288 -2292.

  • P. Bhandari, A.P. Gupta, B. Singh, V.K. Kaul, J. Planar Chromatogr. 19 (2006) 212–215.

    Kaul V.K. , '' (2006 ) 19 J. Planar Chromatogr. : 212 -215.

  • P. Bhandari, N. Kumar, A.P. Gupta, B. Singh, V.K. Kaul, Chromatographia 64 (2006) 599–602.

    Kaul V.K. , '' (2006 ) 64 Chromatographia : 599 -602.

  • N. Kumar, P. Bhandari, B. Singh, A.P. Gupta, V.K. Kaul, J. Sep. Sci. 31 (2008) 262–267.

    Kaul V.K. , '' (2008 ) 31 J. Sep. Sci. : 262 -267.

  • S.K. Choi, M.O. Hyun, S.K. Lee, D.G. Jeong, S.E. Ryu, K.H. Son, D.C. Han, N.D. Sung, N. Baek, Nat. Prod. Res. 20 (2006) 341–346.

    Baek N. , '' (2006 ) 20 Nat. Prod. Res. : 341 -346.

For Submission Guideline click HERE

Manuscript Submission: JPC Manuscript Submission

Senior editors

Editor(s)-in-Chief: B. Spangenberg

Managing Editor: Kakuk Ágnes

Editorial Board

  • T.H. Dzido (Lublin, Poland)
  • Á. M. Móricz (Budapest, Hungary)
  • C.F. Poole (Detroit, MI, USA)
  • E. Reich (Muttenz, Switzerland)
  • B. Renger (Radolfzell, Germany)
  • I. Vovk (Ljubljana, Slovenia)

 

Advisory Board

  • E. BODOKI (Cluj-Napoca, Romania)
  • U. A. TH. BRINKMAN (Amsterdam, The Netherlands)
  • I. CHOMA (Lublin, Poland)
  • V. COMAN (Cluj-Napoca, Romania)
  • W. DAMMERTZ (Kressbronn, Germany)
  • K. FERENCZI-FODOR (Budapest, Hungary)
  • G. INDRAYANTO (Surabaya, Indonesia)
  • R. E. KAISER (Bad Dürkheim, Germany)
  • I. KLEBOVICH (Budapest, Hungary)
  • T. KOWALSKA (Katowice, Poland)
  • L. LEPRI (Florence, Italy)
  • E. MINCSOVICS (Budapest, Hungary)
  • D. NUROK (Indianapolis, IN, USA)
  • B. RENGER (Radolfzell, Germany)
  • J. K. RÓŻYŁO (Lublin, Poland)
  • J. SHERMA (Easton, PA, USA)
  • M. WAKSMUNDZKA-HAJNOS (Lublin, Poland)
  • D. WEDGE (Mississippi, USA)

A. Kakuk - Managing Editor, Akadémiai Kiadó
Address: P.O. Box 245. H-1519 Budapest, Hungary
Fax: +36 1 464 82 95
E-mail: jpc@akkrt.hu

Indexing and Abstracting Services:

  • Science Citation Index
  • Sci Search Research Alert
  • Chemistry Citation Index and Current Content/Physical
  • Chemical and Earth Sciences

 

Journal of Planar Chromatography - Modern TLC
Language English
Size A4
Year of
Foundation
1988
Volumes
per Year
1
Issues
per Year
6
Founder Akadémiai Kiadó
Founder's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
Publisher Akadémiai Kiadó
Springer Nature Switzerland AG
Publisher's
Address
H-1117 Budapest, Hungary 1516 Budapest, PO Box 245.
CH-6330 Cham, Switzerland Gewerbestrasse 11.
Responsible
Publisher
Chief Executive Officer, Akadémiai Kiadó
ISSN 0933-4173 (Print)
ISSN 1789-0993 (Online)

Monthly Content Usage

Abstract Views Full Text Views PDF Downloads
Feb 2021 0 0 0
Mar 2021 5 0 0
Apr 2021 3 0 0
May 2021 11 0 0
Jun 2021 6 0 0
Jul 2021 3 0 0
Aug 2021 0 0 0