A simple, selective, and sensitive thin-layer chromatography (TLC) method is described for the identification and determination of nabumetone in pharmaceutical products. The mobile phase was constituted by the following mixture: n-hexane-chloroform-glacial acetic acid 4:1:1 (ν/ν/ν); and the stationary phase was TLC plate of silica gel 60 F254. The chromatograms were developed and analyzed densitometrically at λ1 = 270 nm and λ2 = 330 nm. RF values and UV spectra were used to identify the compounds. The developed method was characterized by high sensitivity (LOD and LOQ (limits of detection and quantitation) from 0.23 μg per band to 1.00 mg per band), high accuracy (recovery about 100%), and good precision ((relative standard deviation, RSD < 2%).
Polish Pharmacopoeia, 8th edn., Warszawa, 2008.
M. Starek, J. Krzek, Talanta 77 (2009) 925–942.
Krzek J. , '' (2009 ) 77 Talanta : 925 -942 .
P.K. Sahu, M.M. Annapurna, E-J. Chem. 6 (2009) S59–S64.
Annapurna M.M. , '' (2009 ) 6 E-J. Chem. : S59 -S64 .
S.M. Basha, G.D. Rao, Ch.M. Babu, P.B. Rao, Ch.H. Prasad, P.S. Praveen, Int. J. Pharm. Pharm. Sci. 2 (2010) 165–167.
Praveen P.S. , '' (2010 ) 2 Int. J. Pharm. Pharm. Sci. : 165 -167 .
I.F. Al-Momani, Anal. Lett. 30 (1997) 2485–2492.
Al-Momani I.F. , '' (1997 ) 30 Anal. Lett. : 2485 -2492 .
K. Kobylińska, M. Barlińska, M. Kobylińska, J. Pharm. Biomed. Anal. 32 (2003) 323–328.
Kobylińska M. , '' (2003 ) 32 J. Pharm. Biomed. Anal. : 323 -328 .
M. Nobilis, M. Holćapek, L. Kolarova, J. Kopecky, M. Kuneoe, Z. Svoboda, J. Kvetina, J. Chromatogr. 1031 (2004) 229–236.
Kvetina J. , '' (2004 ) 1031 J. Chromatogr. : 229 -236 .
J.D. Ray, R.O. Day, J. Chromatogr. B 336 (1984) 234–239.
Day R.O. , '' (1984 ) 336 J. Chromatogr. B : 234 -239 .
A.J. Vyas, N.A. Aggarwal, B.P. Nagori, J.K. Patel, C.R. Jobanputra, Int. J. Chem. Tech. Res. 2 (2010) 412–418.
Jobanputra C.R. , '' (2010 ) 2 Int. J. Chem. Tech. Res. : 412 -418 .
A.J. Vyas, N.A. Aggarwal, B.P. Nagori, J.K. Patel, C.R. Jobanputra, D.S. Viramgama, Int. J. Chem. Tech. Res. 2 (2010) 543–547.
Viramgama D.S. , '' (2010 ) 2 Int. J. Chem. Tech. Res. : 543 -547 .
A.R. Rote, S.R. Bhalerao, E-J. Chem. 7 (2010) 1463–1467.
Bhalerao S.R. , '' (2010 ) 7 E-J. Chem. : 1463 -1467 .
A.O. Adegoke, S.O. Idowu, A.A. Olaniyi, Afr. J. Med. Sci. 36 (2007) 249–257.
Olaniyi A.A. , '' (2007 ) 36 Afr. J. Med. Sci. : 249 -257 .
Y. Altun, B. Dogan, S.A. Ozkan, B. Uslu, Acta Chim. Slov. 54 (2007) 287–294.
Uslu B. , '' (2007 ) 54 Acta Chim. Slov. : 287 -294 .
S.V. Gandhi, S.S. Ranher, P.B. Deshpande, D.K. Shah, J. Braz. Chem. Soc. 22 (2008) 1068–1072.
Shah D.K. , '' (2008 ) 22 J. Braz. Chem. Soc. : 1068 -1072 .
J. Krzek, M. Starek, Biomed. Chromatogr. 18 (2004) 589–599.
Starek M. , '' (2004 ) 18 Biomed. Chromatogr. : 589 -599 .
M. Starek, J. Krzek, M. Tarsa, M. Żylewski, Chromatographia 69 (2009) 351–356.
Żylewski M. , '' (2009 ) 69 Chromatographia : 351 -356 .
ICH Guideline Q2(R1) on Validation of Analytical Procedures: Text and Methodology, International Conference on Harmonization, 6 Nov 1996, incorp. Nov 2005, Geneva.